盐酸千金藤碱对急性肝损伤小鼠保护作用的研究  

A study on hepatoprotective effects of Cepharanthine Hydrochloride on acute liver injury in mice

作　　者：王晓丽[1,2] 郑立运[1,2] 张艳[1,2] 王宁[1,2] 江金花[1,2] 马方[1,2] 王庆端[1,2] 

机构地区：[1]河南省郑州大学医药科学研究院药化研究室,郑州450052 [2]河南省肝病药理重点实验室,郑州450052

出　　处：《中华中医药杂志》2009年第10期1384-1387,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：河南省杰出人才创新基金资助项目(No.0521002200)

摘　　要：目的:探讨盐酸千金藤碱(CH)对急性肝损伤小鼠的保护作用及其机制。方法:建立四氯化碳(CCl4)和D-氨基半乳糖(D-GalN)致急性肝损伤小鼠模型,设CH大、中、小剂量组,阳性对照组(阿拓莫兰),空白对照组和模型对照组。观察CH对小鼠血清转氨酶、一氧化氮(NO)、一氧化氮合酶(NOS)、肝组织氧化指标及肝脏病理学的影响。结果:CH可明显降低CCl4和D-GalN所致肝损伤小鼠的血清转氨酶活性,增强肝脏超氧化物歧化酶(SOD)活性,减少丙二醛(MDA)和NO的含量,降低NOS活性;并缩小肝脏坏死范围。结论:CH对CCl4和D-GalN所致肝损伤有良好的肝保护作用,其机制可能是提高SOD活性、清除自由基,降低NO含量,减少其对细胞和组织的损伤。Objective：To explore the effect of Cepharanthine Hydrochloride （CH） on acute liver injury in mice caused by carbon tetrachloride （CCl4） or D-galactosamine （D-GalN）. Methods： Mice were given different dose of CH prior to CCl4 or D-GalN, and ATOMOLAN was chose as positive drug. ALT and AST activities in serum, SOD and NOS activities in liver, MDA and NO contents in liver, and histopathological examination were detected to study the hepatoprotective effect of CH. Results： CH showed significant protection against the hepatotoxic actions of CCl4 and D-GalN, markedly alleviated aspartate aminotransferase （AST）, alanine aminotransferase （ALT） level in serum, enhanced superoxide dismutase（SOD）, nitric oxide synthase（NOS） activity in liver, reduced Maeic Dialdehyde （MDA）, nitric oxide（NO） content. Histochemical investigation showed that CH diminuted necrosis extent and degree in liver tissue, and reduced hydropic degeneration. Conclusion： CH protects against CCl4 and D-GalN induced liver injury in mice, and the mechanism of CH might be enhancing antioxidant enzyme activities, removing free radical and reducing NOS activites.

关 键 词：盐酸千金藤碱 四氯化碳 D-氨基半乳糖 急性肝损伤 

分 类 号：R285.5[医药卫生—中药学]