中等强度运动后大鼠不同部位组织的荧光光谱研究  

A Study the Autofluorescence Spectrum of Tissues in Different Places of Rats after Mid-intensity Exercise

作　　者：任文君[1] 张斌南[2] 徐正红[3] 张镇西[1] 李政[1] 

机构地区：[1]西安交通大学生命科学与技术学院,陕西西安710049 [2]西安交通大学体育部,西安陕西710049 [3]西安交通大学电气工程学院,西安陕西710049

出　　处：《北京体育大学学报》2009年第8期51-53,共3页Journal of Beijing Sport University

基　　金：国家自然科学基金资助(60378018和60578026);西安交通大学校长基金资助(sk0816;SK0817)

摘　　要：为了研究运动训练大鼠不同部位组织(重点是脂肪组织)和器官的自体荧光光谱特性。方法:通过激光诱导荧光光谱技术测量一次中等强度力竭运动后大鼠组织的自体荧光光谱,并在肠系膜部位的脂肪组织的三维荧光光谱中发现了和运动相关的特有荧光峰,并判别出此荧光峰对应的荧光物质是NADH(还原态烟酰胺腺嘌呤二核苷酸),且研究了运动状态与峰强的相关性。结果:中等强度运动后大鼠肠系膜部位的脂肪组织的能量代谢强于大网膜部位的脂肪组织、皮下脂肪组织,并且强于前腿肌肉、比目鱼肌及其它脏器;NADH自体荧光光谱特性是判断机体组织代谢程度的有效的指标之一。Aim： to investigate three-dimensional autofluorescence spectral characteristics of tissues and organs of different places of exercising rats. Methods： By laser-induced fluorescence （LIF） technique, three-dimensional autofluorescence spectral characteristics of tissues and organs of different places of exercising rats after treadmill exercise with 5° incline, 32m/min, 180 min/time are studied. Results： Specific exercise-related fluorescence peaks are discovered in the three-dimensional fluorescence spectra of adipose tissue and fluorescent substance corresponding to the fluorescence peaks is determined to be nicotine-amide adenine dinucleotide reduced（NADH）. The study indicates that the energy metabolism of rats＇ adipose tissue is stronger than other organs and the autofluorescence spectral characteristic is one of the effective indexes to judge the metabolic of muscle.

关 键 词：自体荧光 荧光光谱 NADH 不同部位 脂肪组织 

分 类 号：G804.7[文化科学—运动人体科学]