维药神香草对哮喘大鼠血清嗜酸性粒细胞趋化因子和可溶性P选择素的影响  

Effect of Uygur Medicine Hyssopus officinalis L. on Serum Eotaxin-2,Eotaxin-3 and sP-selectin Level of Asthma Rats

作　　者：侯敏[1,2] 马秀敏[1,2] 丁剑冰[1,2] 朱明[2] 马骏[3] 马雪萍[1] 王亚男[2] 田树革[1] 

机构地区：[1]新疆医科大学；新疆名医名方与特色方剂学重点实验室,乌鲁木齐830011 [2]新疆医科大学基础医学院,乌鲁木齐830054 [3]新疆医科大学附属中医医院,乌鲁木齐830000

出　　处：《科技导报》2009年第19期90-93,共4页Science & Technology Review

基　　金：新疆维吾尔自治区自然科学基金项目(200821129);新疆维吾尔自治区教育厅重点项目(XJEDU2008I17);新疆高技术研发项目(PT0708)

摘　　要：通过检测维吾尔药神香草对哮喘大鼠血清嗜酸性粒细胞趋化因子-2(Eotaxin-2)、嗜酸性粒细胞趋化因子-3(Eotaxin-3)和可溶性P选择素(sP-selectin)的影响,探讨神香草治疗哮喘的作用机制。采用的方法是,将大鼠随机分为正常对照组、哮喘模型组、地塞米松治疗组、神香草高剂量治疗组和低剂量治疗组。采用卵清白蛋白(OVA)、氢氧化铝及百白破疫苗联合致敏和OVA生理盐水雾化激发的方法制备哮喘模型。计数各组大鼠支气管肺泡灌洗液(BALF)中嗜酸性粒细胞(EOS)的个数;采用酶联免疫吸附试验(ELISA)法检测大鼠血清Eotaxin-2、Eotaxin-3和sP-selectin的水平。结果显示,与模型组相比,各治疗组大鼠BALF中EOS的个数明显减少,血清Eotaxin-2的水平明显下降,差异有统计学意义(P<0.05);与神香草低剂量治疗组相比,高剂量治疗组大鼠BALF中EOS的个数明显减少,血清Eotaxin-2的水平明显下降,差异有统计学意义(P<0.05)。各组大鼠BALF中EOS的数量与血清Eotaxin-2水平密切相关(r=0.829),与血清Eotaxin-3和sP-selectin水平均呈正相关(r=0.635,0.348),P<0.05。各组大鼠血清Eotaxin-2与Eotaxin-3和sP-selectin水平呈正相关(r=0.447,0.362),P<0.05。由此推测,Eotaxin-2、Eotaxin-3和sP-selectin可相互协同,加剧哮喘的炎症反应,神香草可能通过抑制Eotaxin-2的分泌,减轻哮喘的炎症反应。The Uygur medicine Hyssopus officinalis L. on serum Eotaxin-2, Eotaxin-3 and sP-selectin levels of asthma rats is studied for the mechanism of its treatment of asthma. The test rats were randomly divided into normal control group, asthma model group and dexamethasone group and Hyssopus officinalis L. high and low dose group. They were sensitized with OVA, Al（OH）3 and DPT vaccine, and then were challenged with inhalation of aerosolized OVA solution for preparation of asthma model. The levels of serum Eotaxin-2, Eotaxin-3 and sP-selectin were determined with ELISA. The results show that as compared with model group, the number of EOS in BALF of the treatment groups is significantly reduced, and serum levels of Eotaxin-2 are decreased significantly （P〈O.05）. Compared with Hyssopus officinalis L. lowdose treatment group, the number of EOS in BALF is significantly reduced for the high dose group, and the serum levels of Eotaxin-2 are decreased significantly （P〈0.05）. The number of EOS in BALF and the serum levels of Eotaxin-2 are closely related （r=0.829）. The EOS has a positive relationship with the serum Eotaxin-3 and sP- selectin levels （r=0.635, 0.348）, P〈0.05. The serum Eotaxin-2 level has a positive relationship with the Eotaxin-3 sP-selectin levels （r= 0.447, 0.362, P〈0.05）. It is concluded that Eotaxin-2, Eotaxin-3 and sP-selectin may be in a mutual coordination, to aggravate the inflammatory response of asthma. Hyssopus officinlais L. may inhibit the secretion of Eotaxin-2, reducing the inflammatory response of asthma.

关 键 词：神香草 哮喘 嗜酸性粒细胞趋化因子 可溶性P选择素 

分 类 号：R29[医药卫生—民族医学]