大鼠胰岛移植物制备与异种移植  

Preparation of rat islet transplant and xenotransplantation

作　　者：周茂华[1,2] 陈东明[1,2] 唐军民[1,2] 夏兆骥[1,2] 

机构地区：[1]中国武警医学院外科教研室 [2]北京医科大学第三临床医学院成形外科研究中心

出　　处：《中华器官移植杂志》1998年第4期200-201,共2页Chinese Journal of Organ Transplantation

基　　金：国家自然科学基金

摘　　要：增加胰岛收获量，提高胰岛纯度一直是胰岛移植中面临的重要问题，本实验经胰管注射胶原酶、胰腺静止消化分离成年大鼠胰岛，葡聚糖离心纯化。纯化后胰岛收获量为６１０～８２０个／胰腺，纯度达９２％；胰岛形态结构完整，内分泌细胞超微结构保持良好；对葡萄糖刺激反应胰岛素释放量是基础分泌水平的８倍；Rat islets were isolated with in situ ductal collagenase distention, stationary digestion and dextran gradient separation. After purification, about 610￣820 islets/pancreas were obtained, with an average purity of 92% islets. The isolated islets demonstrated a well preserved endocrine cell ultrastructure, responded to glucose stimulation with an eight fold increase of insulin secretion over basal levels, and temporarily reversed experimental diabetes in mice. This method produced a high yield of viable purified rat islets.

关 键 词：胰岛移植 分离 纯化 异种移植 移植物 

分 类 号：R657.5[医药卫生—外科学]