广东汉人HLA-DRB_1基因的PCR-SSP定型分析及其对风湿热遗传易感性的研究  

HLA DRB 1 GENES INVOLVED IN THE GENETIC SUSCEPTIBILITY TO RHEUMATIC FEVER IN GUANGDONG HANS

作　　者：赵长林[1] 余步云[1] 古洁若[1] 陈国庆[1] 

机构地区：[1]中山医科大学附属第三医院

出　　处：《中华风湿病学杂志》1998年第3期164-167,共4页Chinese Journal of Rheumatology

摘　　要：目的：研究ＨＬＡＤＲＢ１基因对风湿热的遗传易感性。方法：用ＰＣＲＳＳＰ方法对广东地区５１例风湿热患者和１０２位正常人的ＨＬＡＤＲＢ１基因进行定型分析且探讨ＨＬＡＤＲＢ１等位基因与风湿热的相关性。结果：风湿热患者ＨＬＡＤＲＢ１等位基因分布异常，其中，ＤＲＢ１０３０１基因频率为２３３％，而正常人仅为６５９％（校正Ｐ＜００５，相对危险率为４６７）。Objective:To determine whether genetic factors could be involved in the pathogenesis of rheumatic fever.Methods:The HLA DRB 1 alleles were typed by PCR amplification with sequence specific primers (PCR SSP).Fifty one patients with rheumatic fever and 102 normal individuals were analysed by this method.And the correlation between HLA DRB 1 allele and rheumatic fever was also studied.Results:The allele of DRB 1 0301 was present in 23 3% of patients with rheumatic fever compared with 6 59% of normal control subjects (corrected P <0 05;relative risk=4 67).Conclusion:DRB 1 0301 allele is positively associated with rheumatic fever.

关 键 词：风湿热 等位基因 HLA-DRB1 PCR 遗传易感性 

分 类 号：R593.210.2[医药卫生—内科学]