南海常见硬骨鱼类COⅠ条码序列  

COⅠ BARCODING SEQUENCES OF TELEOSTS IN THE SOUTH CHINA SEA

作　　者：王中铎[1,2] 郭昱嵩[1] 陈荣玲[1] 何晓莹[1] 刘楚吾[1,2] 刘筠[2] 

机构地区：[1]广东海洋大学水产学院,湛江524066 [2]湖南师范大学生命科学院,长沙410081

出　　处：《海洋与湖沼》2009年第5期608-614,共7页Oceanologia Et Limnologia Sinica

基　　金：“十一五”国家科技支撑计划项目,2007BAD29B03号;广东省自然科学基金资助项目,8452408801001272号

摘　　要：运用一对特异性引物扩增并测定了南海硬骨鱼类40个物种89个样本的线粒体DNA的细胞色素c氧化酶Ⅰ(COⅠ)约660bp的部分序列,即COⅠ条码序列。综合比较了《中国鱼类系统检索》、《海洋鱼类志》、FishBase鱼类形态学分类库、ITIS综合分类学信息系统和生物条码系统(The Barcode of Life Data System,BOLD)的相应形态学与DNA序列资料,在揭示完善我国鱼类检索系统必要性基础上,探讨了COⅠ条码序列在硬骨鱼类辅助物种鉴别和适用性,并对运用该条码序列库完善我国鱼类检索系统的可能途径进行了初步摸索。结果表明,COⅠ条码序列获取便捷,广泛适用硬骨鱼类物种鉴别,并可用于低级分类阶元的系统进化分析。本研究结果可为分子生物学辅助分类、市场监督、资源有序利用和保护提供参考。Forty species of teleosts in the South China Sea were sequenced （barcoded） in a 660bp region of mitochondrial cytochrome oxidase subunit I gene （CO I ）. Most of the species were represented by multiple specimens, and 89 sequences were generated. The average intra-species, -genus, -family, and -order of Kimura two parameter （K2P） distances are 0.0084, 0.1567, 0.1839 and 0.2319, respectively. All the species could be differentiated in CO I sequence, despite of single individuals of any two species had haplotypes characteristic of a congener. Although DNA barcoding aims to develop species identification systems, some phylogenetic signals are apparent in the data. In the neighbour-joining tree for all 89 sequences, species of a genera clustered invariably in three major clusters： snappers, groupers, and the other fishes. The former two clades revealed after bootstrapping generally corresponded well with expectations. It is concluded that CO I sequencing, or ‘barcoding＇, can be used to identify fish species.

关 键 词：DNA条码 COⅠ 物种鉴别 硬骨鱼类 

分 类 号：Q959[生物学—动物学]