内耳免疫反应时血清及外淋巴液IL-6水平的测定及其意义  

Determinatiou of IL-6 in serum and perilymph during inner ear immune response

作　　者：曾新力[1] 龚树生[1] 

机构地区：[1]同济医科大学附属协和医院耳鼻咽喉科

出　　处：《临床耳鼻咽喉科杂志》1998年第11期513-516,共4页Journal of Clinical Otorhinolaryngology

基　　金：国家自然科学青年基金!39400146

摘　　要：为了解在用keyholelimpethemocyanin（KLH）引发的大鼠继发性内耳免疫反应时，实验动物血清及外淋巴液中是否有细胞因子活性的变化，采用免疫测定方法对实验动物血清及外淋巴液中白细胞介素（interleukin－6，IL－6）活性进行测定。结果：在发生继发性内耳反应初始，外淋巴液未检测到IL－6，而6h后外淋巴液中检测到了IL－6的最早水平，24h后IL－6水平达到最高峰。此后逐渐减少，但7d后在外淋巴液中仍可测定IL－6。在观察期对照内耳的外淋巴液中仅检测到低水平的IL－6，72h后就消失。血清中IL－6水平无变化。提示该检测不仅提供了支持存在有受淋巴因子调控的内耳免疫反应，而且也支持调控ICAM－1表达的细胞因子，也可能还来自内淋巴囊以外的其他部位。During secondary iuner ear immune responses against keyhole limpet hemocyanin (KLH),to understand the change of interleukin-6 (IL-6) levels in perilymph and serum, IL-6 lev-els in perilymph and serum were investigated using an immunoassay. No IL-6 levels were detectable at Day o in perilymph. The earliest perilymph IL-6 levels were observed after 6 h,peaking at 24 h,and then decreasing gradually. IL-6 level reninants were detected at 7 days in perilymph. In con-trast,during this observatinn period,low IL-6 levels were only detectable in perilymph from control ears,disappearing after 72 h. IL-6 levels dont change in serum. Previous study has identified the endolymphatic sac (ES) lags behind the earlier appearance of intercellular adhesion molecule-l (I-CAM-1) in the spiral modiolar vein and spiral ligament during immune response. The present study not only provides further support for the existence of an inner ear immune response which is regu-lated by cytokines,but also supports that cytokines controlling expression of adhesion molecules are released probably by cells which located out of ES.

关 键 词：内耳 免疫反应 外淋巴液 血清 白细胞介素6 

分 类 号：R392.11[医药卫生—免疫学]