CpG寡脱氧核苷酸刺激培养对慢性淋巴细胞白血病细胞染色体分析的影响  

CpG-oligodeoxynucleotide stimulation improves the successfulness for karyotypic analysis of chronic lymphocytic leukemia cells

作　　者：刘琼[1] 徐卫[1] 仇海荣[1] 王蓉[1] 于慧[1] 范磊[1] 缪扣荣[1] 李建勇[1] 

机构地区：[1]南京医科大学第一附属医院,江苏省人民医院血液科,210029

出　　处：《中华血液学杂志》2009年第9期601-604,共4页Chinese Journal of Hematology

基　　金：国家自然科学基金（30871104）;江苏省医学领军人才基金（LJ200623）;江苏省医学重点人才基金（RC2007042）;汀苏省自然科学基金（BK2007249）;南京市科技发展项目（303070164JA08）;江苏省“青蓝工程”中青年学术带头人培养对象项目;江苏省“六大人才高峰”资助项目;江苏省“333工程”项目（RS07）

摘　　要：目的探索应用CpG寡脱氧核苷酸刺激对慢性淋巴细胞白血病（CLL）细胞染色体分析的影响。方法对70例CLL患者的外周血（或骨髓）标本，分别应用植物血凝素、CpG寡脱氧核苷酸和CpG寡脱氧核苷酸联合IL-2刺激培养72h，常规收获细胞制备染色体并进行R显带分析。应用组合探针进行间期细胞荧光原位杂交检测。结果植物血凝素组、CpG寡脱氧核苷酸组和CpG寡脱氧核苷酸联合IL-2组染色体分析成功率（可分析分裂象≥20个）分别为90．0％、68．6％和68．6％，异常核型检出率分别为3．2％、43．6％和43．6％，组合探针荧光原位杂交异常核型检出率为64．3％。结论应用CpG寡脱氧核苷酸刺激培养CLL细胞，可提高染色体异常检出率。Objective To explore the effect of CpG-oligodeoxynucleotides （ODN） in chromosome study of chronic lymphocytic leukemia （CLL）. Methods Blood or bone marrow cells of 70 CLL patients were cultured for 72 h with PHA , CpG-ODN and CpG-ODN combined with IL-2, respectively. Routine karyotype analysis with R banding technique and interphase fluorescence in situ hybridization （FISH） were performed. Results The metaphase number ≥ 20 was considered as successful stimulation, which in PHA, CpG-ODN and CpG-ODN combined IL-2 groups were 90.0% , 68.6% and 68.6%, respectively, and the detection rates of chromosome aberrations were 3.2% , 43.6% and 43.6% , respectively. The aberrations rates detected by interphase FISH with a panel of probes was 64.3%. Conclusion CpG-ODN DSP30 can effectively raise the detection rate of chromosome aberrations in CLL patients.

关 键 词：白血病 淋巴细胞 慢性 细胞遗传学 CPG寡脱氧核苷酸 荧光原位杂交 

分 类 号：R686[医药卫生—骨科学]