结直肠癌线粒体DNA拷贝数与其微卫星不稳定关系的研究  

The relationship between copy number and microsatellite instability of mitochondrial DNA in colorectal cancer

作　　者：黄平[1] 张鋆歆[2] 赵婷[1] 

机构地区：[1]解放军第三○五医院消化内科,北京100017 [2]解放军第三○五医院病理科,北京100017

出　　处：《中华内科杂志》2009年第10期837-840,共4页Chinese Journal of Internal Medicine

基　　金：全军“十五”医药卫生科研基金课题（01MA215）

摘　　要：目的探讨结直肠癌（CRC）的线粒体DNA（mtDNA）拷贝数异常与临床指标及微卫星不稳定（MSI）的关系。方法选取50例CRC及相应癌旁组织标本，分别提取基因组DNA。对非编码区微卫星位点进行测序；对线粒体ND1基因进行荧光定量PCR，进而计算出mtDNA拷贝数；然后与临床指标和非编码区MSI进行比较分析。结果CRC组织的mtDNA平均拷贝数／细胞数为312±185，而相应的癌旁组织为525±125，前者显著低于后者（P〈0．001）。mtDNA拷贝数高低与非编码区MSI有明显相关性（P〈0．001），与性别、年龄、病理分型、TNM分期无相关性（P〉0．05）。结论CRC的mtDNA拷贝数明显降低，这种降低与线粒体的MSI相关。Objective To study the relationship between the abnormality of mitochondrial DNA （mtDNA） copy number and the clinical parameters and microsatellite instability （MSI） in colorectal cancer. Methods Total DNA was extracted from cancer and pericancer tissue from 50 colorectal cancer （CRC） biopsy samples. Non-coding region sequencing was done and the copy number of mtDNA was quantitated with real-time PCR in mitochondrial ND1 gene. The relationship between clinical indicators, mtMSl and mitochondrial copy number was detected. Results The mean copy number of mtDNA 312 ± 185 in the tumor tissue was significantly lower than that 525 ± 125 of the corresponding non-tumor tissue of these patients （ P 〈 0. 001 ）. No significant correlation was found between mtDNA copy number and other variables including age, gender, pathological type and clinical stage （P 〉 0. 05 ）. However, there was a significant correlation between copy number and mtMSI （P 〈 0. 001 ）. Conclusion There is a significant reduction of mtDNA in CRC patients, which may be caused by mtMSI.

关 键 词：结直肠肿瘤 微卫星不稳定 DNA 线粒体 拷贝数 实时定量PCR 

分 类 号：R686[医药卫生—骨科学]