鼻息肉黏膜上皮钠通道蛋白表达的研究  

Expression of epithelial Na^＋ channel in human nasal polyp

作　　者：江一鸣[1] 李吉平[1] 张淳[1] 王家东[1] 姜叙诚[2] 

机构地区：[1]上海交通大学医学院仁济医院耳鼻咽喉头颈外科耳鼻咽喉科研究所,200001 [2]上海交通大学基础医学院病理学教研室

出　　处：《中华耳鼻咽喉头颈外科杂志》2009年第10期861-865,共5页Chinese Journal of Otorhinolaryngology Head and Neck Surgery

摘　　要：目的比较鼻息肉与正常中鼻甲黏膜上皮钠通道（epithelial sodium channels，ENaC）蛋白的表达，探讨鼻黏膜ENaC蛋白依赖的Na^＋吸收机制及液体转运机制。方法12例Ⅱ型2期慢性鼻-鼻窦炎（chronic rhinosinusitis，CRS）患者为鼻息肉（nasal polyp，NP）组，5例行鼻中隔矫正术和上颌窦囊肿摘除术患者的中鼻甲黏膜（ethmoid cornu mucosa，ECM）为对照组，内镜下取新鲜组织制成冰冻切片后行免疫荧光染色，激光共聚焦显微镜下记录荧光相对表达量；反转录-实时聚合酶链反应（RT real—time PCR）法检测两组ENaC-α、β、γ mRNA的表达。结果NP组ENaC蛋白免疫荧光相对表达量（x±5）为35．79±5．47，高于ECM组（22．17±5．43），差异具有统计学意义（t=4．69，P〈0．01）；NP组ENaC-α、β、γ mRNA表达（分别为2．06±0．42、1．97±0．32、1．96±0．54）均大于ECM组（分别为1．01±0．10、0．98±0．08、0．97±0．06），差异具有统计学意义（t值分别为5．482、6．659、4．036，P值均〈0．01）；ENaC三个业基mRNA的表达在NP组与ECM组均为α〉β〉γ；NP组ENaC蛋白的相对表达量与ENaC-α、β、γ mRNA的表达呈正相关（r值分别为0．907、0．948、0．919，P值均〈0．01）。结论ENaC蛋白及ENaC—α、β、γ mRNA在鼻息肉黏膜中的表达上调，且二者具有同步性，ENaC蛋白表达上调可能是造成黏膜下水肿，最终导致鼻息肉形成的重要原因之。Objective To evaluate the expression of epithelial sodium channel in nasal polyps and normal nasal mucosa and to characterize the ENaC-mediated Na^＋ absorption and the mechanism of the liquid transport in human upper airway epithelia. Methods The tissue from 12 patients with nasal polyps （NP） and the normal ethmoid cornu mucosa（ECM） from 5 patients were obtained through endoscopic surgery. The expression of ENaC was detected by Immunofluorescence and the concentration of ENaC α、β、γ-mRNA were detected by RT real-time PCR. Results The ENaC in NP group （ 35.79± 5.47 ） was higher than that in ECM group（22.17 ±.43, t = 4. 687, P 〈 0. 01 ）. The expression of ENaC-α,β,γ mRNA in NP group （ respectively 2. 06 ± 0.42, 1.97 ± 0. 32, 1.96 ± 0. 54 ） was higher than that in ECM group （ respectively 1.01 ±0. 10, 0.98 ±0.08, 0.97 ±0.06; t =5. 482, 6.659, 4.036, all P 〈0.01）. The mRNA expression of three subunits of ENaC was as follows ： α〉β〉γ in both groups. The mRNA expression of α,β,γ subunit of ENaC in NP group was higher than that in ECM group （ P 〈 0. 01 ）. The expression of three subunits of ENaC was correlated respectively with that of mRNA in NP group. Conclusions The up-regulation of ENaC in human NP was associated with the expression of ENaC mRNA, that made hydropsia and might be one of the most important cause of the develop of nasal polyp.

关 键 词：鼻息肉 鼻黏膜 钠通道 

分 类 号：R686[医药卫生—骨科学]