人血单核细胞和U937细胞产生IFN-α的比较  

Comparison of IFN-α Production in Human Blood Monocytes and U937 Cells

作　　者：孙路虹[1] 季晓辉[1] 姚堃[1] 李焕娣[1] 丁如宁[1] 

机构地区：[1]南京医科大学微生物学教研室,南京210029

出　　处：《上海免疫学杂志》1998年第5期287-288,294,共3页Shanghai Journal of Immunology

摘　　要：一般认为人体单核-巨噬细胞(Mo-Mφ)系在体外诱生IFN-α较为困难,本文试图找到适合的培养条件,使Mo-M(?)系能在体外诱生IFN-α.结果显示:经M-CSF长期预处理和IFN-γ的短期预处理或单用IFN-γ短期预处理后,NDV刺激均能使人外周血Mo-M(?)产生较高水平的IFN-α;U937细胞只能产生低水平的IFN-α.It is generally held that the production of IFN-γ by human monocyte-macrophage system in vitro is rather difficult. We attempted to investigate the optimal culture conditions for monocyte-macrophage system to produce IFN-α. The results showed that after prolonged M-CSF pretreatment with short duration with IFN-γ pretreatment, or with IFN-γ pretreatment alone, the monocyte-macrophage system could produce high level of IFN-α by NDV stimulation;but the U939 cells only produce low level of this cytokine.

关 键 词：单核细胞 U937细胞 干扰素Α 

分 类 号：R392.12[医药卫生—免疫学] R392.11[医药卫生—基础医学]