白果内酯对谷氨酸兴奋毒性致神经损害的保护作用  

Neuroprotective effect of bilobalide against neuronal damage by glutamate-induced excitotoxicity

作　　者：徐静[1] 孙长凯[2] 王冬梅[1] 徐红[1] 张健[2] 张玉梅[2] 马辉[2] 王禄[2] 吴兰香[2] 

机构地区：[1]大连医科大学机能学实验室,辽宁大连116044 [2]大连医科大学脑疾病研究所,辽宁大连116044

出　　处：《中草药》2009年第10期1593-1597,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30400143)

摘　　要：目的观察不同剂量白果内酯对谷氨酸致海马神经元损害的影响,以探讨白果内酯在抗兴奋毒性神经损害中的应用价值。方法原代培养新生SD大鼠海马神经元,建立谷氨酸诱导的兴奋毒性模型;采用台盼蓝染色、TUNEL染色凋亡神经元测定及乳酸脱氢酶(LDH)活性测定的方法观察不同剂量白果内酯的神经保护作用,并与谷氨酸NMDA(N-甲基-D-天门冬氨酸盐)受体非竞争性拮抗剂MK-801的神经保护作用相比较。结果在一定剂量范围内,白果内酯可提高谷氨酸损伤的海马神经元细胞的存活率、降低细胞凋亡率、减少细胞中LDH的漏出,具有剂量依赖性,于100μmol/L剂量下呈现最佳神经保护效果,但弱于MK-801(浓度为10μmol/L)。结论白果内酯对谷氨酸诱导的兴奋毒性神经损害具有保护作用。Objective To observe the effect of bilobalide in different administration modes against glutamate-induced neuronal damage so that their application value was determined. Methods Based on glutamate-induced excitotoxicity to primarily culture hippocampal neuron of neonatal Sprague-Dawley （SD） rat, trypan blue, TUNEL, and LDH were utilized in our experiment to study the protective effect of bilobalide on neuron in different doses, as well as to compare with the NMDA receptor uncompetitive antagonist- MK-801. Results Bilobalide could increase cell survival rate, reduce apoptosis rate, and decrease LDH leakage in different degrees and in a dose depend manner within the certain range. The maximal protection of bilobalide was achieved at a concentration of 100 μmol/L, but inferior to MK-801 （10 μmol/L）. Conclusion Treatment with bilobalide could protect the neurons against glutamate-induced excitotoxicity.

关 键 词：白果内酯 谷氨酸 海马神经元 

分 类 号：R286.1[医药卫生—中药学]