芹菜素对大鼠急性脑损伤的保护作用  

Protective effect of apigenin in acute cerebral injury of rats

作　　者：刘婵[1] 涂丰霞[1] 陈翔[1] 

机构地区：[1]温州医学院附属第二医院暨育英儿童医院康复中心和脑科中心,浙江温州325000

出　　处：《中草药》2009年第10期1598-1602,共5页Chinese Traditional and Herbal Drugs

基　　金：浙江省教育厅资助项目(20020448)

摘　　要：目的研究芹菜素对大鼠脑匀浆铁依赖性脂质过氧化(IDLPO)的抑制作用以及对急性脑缺血-再灌注大鼠脑损伤的保护作用。方法体外实验用Fe2+诱导大鼠脑匀浆IDLPO反应,TBA试验测定不同质量浓度芹菜素和去铁胺干预的脑匀浆丙二醛(MDA)水平。体内实验采用改良大脑中动脉线栓法建立急性局灶性大鼠脑缺血模型,对大鼠进行神经行为学评分、脑组织TTC染色及电镜观察,观察芹菜素对不同再灌注时间(24、48、72h)脑组织MDA、超氧化物歧化酶(SOD)的影响。结果芹菜素能使大鼠脑匀浆的MDA水平显著下降(P<0.01);与去铁胺组比较无显著性差异(P>0.05)。体内实验模型组和芹菜素组大鼠均出现不同程度的神经行为学评分异常,TTC染色出现额颞顶叶和尾壳核梗死灶,电镜下模型组病变侧细胞内及间质水肿,呈空泡化,神经细胞均有核固缩表现;芹菜素组病变较之明显减轻。与假手术组比较,模型组和芹菜素组各时间点MDA水平均有升高(P<0.01、0.05),模型72h组较模型24、48h组明显升高(P<0.01、0.05),芹菜素48、72h组与相应模型组比较明显降低(P<0.01、0.05);模型组和芹菜素组的SOD活性与假手术组比较显著降低(P<0.01、0.05);芹菜素组(除24h组)较相应模型组显著升高(P<0.05)。结论芹菜素对大鼠急性脑缺血-再灌注损伤有保护作用,其机制可能与抑制IDLPO作用、螯合脑组织Fe2+及激活脑组织SOD的活性有关。Objective To study the inhibition of iron-dependent lipid peroxidation （IDLPO） by apigenin on rat cerebral homogenate in vitro and to evaluate the neuroprotective effects of apigenin on acute transient focal cerebral ischemia-reperfusion injury in rats. Methods In vitro IDLPO on rat cerebral homogenate was induced with ferrous sulfate, the incubation mixture was observed at three various concentration of apigenin and deferoxamine （DFX）, and malondialdehyde （MDA） level was assayed by the 2-thiobarbituric acid （TBA） test. In vivo, the transient focal cerebral isehemia-reperfusion model in rats was established with insertion of thread embolish into middle cerebral artery. In experiment groups the neurological behavior scores, TTC stain of brain slices, and neurocyte morphology were observed. The homogenate of left hemisphere was collected for investigating the effect of apigenin on content of MDA and activity of superoxide dismutase （SOD） in 24, 48, and 72 h. Results MDA was reduced in three various concentration of apigenin on rat cerebral homogenate （P〈0.01）, but compared with DFX there was no significant difference （P〈0.05）. In vivo, abnormal neurological behavior scores existed and typical cortical infarct lesions were found by TTC stain in both apigenin and model groups. An obvious intraeellular and intercellular edema and vacuolization were found in the cerebral cortexes and hippoeampuses in model group. There was karyopycnosis in glias and neurons. However lesion was alleviated in apigenin group. The MDA contents in both apigenin and model groups were increased greatly compared to ones of Sham-operated group, respectively （P〈0. 01 and 0.05）. MDA in mode1 group for 72 h increased greatly compared to ones in model group for 24 and 48 h （P〈0.01 and 0.05）. MDA was remarkabaly lower in apigenin group for 48 and 72 h than ones in model group, respectively （P〈0.01 and 0.05）. The SOD activities were degraded significantly in model and apigenin groups compared to one o

关 键 词：芹菜素 铁依赖性脂质过氧化 脑缺血-再灌注损伤 

分 类 号：R286.10[医药卫生—中药学]