白芷种质资源遗传多样性的ISSR研究  

Genetic diversity of Radix Angelicae Dahuricae germplasmic resource based on ISSR analysis

作　　者：郭丁丁[1] 马逾英[1] 唐琳[2] 陈要臻[2] 吕强[1] 

机构地区：[1]成都中医药大学药学院,四川成都610075 [2]四川大学生命科学学院,四川成都610064

出　　处：《中草药》2009年第10期1627-1630,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30572337)

摘　　要：目的应用ISSR分子标记技术分析中药白芷的遗传多样性。方法对4大类商品白芷及白芷近缘野生种兴安白芷共20个居群的白芷种质进行ISSR分析,利用NTSYS2.1e软件分析遗传相似系数,UPGMA方法聚类,构建亲缘关系系统图。结果9条引物共得到97条扩增条带,其中多态性条带37条,占38%;20个居群的样品聚为两大类。结论不同产地栽培白芷的遗传多样性水平较低,种质间的亲缘关系较近;而兴安白芷与4大类商品白芷存在遗传差异。Objective To determine the genetic diversity of Radix Angelicae Dahuricae by ISSR. Methods The 20 populations of germplasmic resources of Radix Angelicae Dahuricae including four commodity varieties and Radix Angelicae Dahuricae were analyzed by ISSR. To make up the systematic diagram of genetic relationship by NTSYS2. le software and cluster by UPGMA method. ResuLts A total of 97 ISSR bands from nine primers were obtained, among which 37 were polymorphic bands. The average percentage of polymorphic bands was 38 %. The 20 populations of germplasmic resources of Radix Angelicae Dahuricae were classified into two large groups. Conclusion The level of germplasm diversity of Argelica dahurica from various habitats is lower and the affinity relationship of Radix Angelicae Dahuricae is closer. But there is genetic difference between Angelica dahurica and the four commodity varieties.

关 键 词：白芷 ISSR 种质资源 遗传多样性 

分 类 号：R282.7[医药卫生—中药学]