黄连遗传多样性的ISSR分析  

ISSR Analysis for genetic diversity of Coptis chinensis

作　　者：张春平[1] 何平[1] 胡世俊[2] 王瑞波[1] 张益锋[1] 刘长坤[1] 高姗[1] 

机构地区：[1]西南大学生命科学学院三峡库区生态环境教育部重点实验室,重庆市三峡库区植物生态与资源重点实验室,重庆400715 [2]中国科学院昆明植物研究所,云南昆明650204

出　　处：《中草药》2009年第10期1630-1634,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30070080)

摘　　要：目的对野生黄连进行遗传多样性研究。方法通过ISSR技术对7个野生黄连居群共78个个体进行遗传多样性分析。结果用12个随机引物共扩增出106条清晰条带,其中72条具多态性,平均多态性位点比率为67.92%,Nei′s基因多样性指数H=0.1803,Shannon多样性指数I=0.2832,遗传分化指数Gst=0.6815,遗传距离和遗传一致度分别为:0.0894～0.1846和0.8321～0.9127。结论黄连种水平上具有较高的遗传多样性,遗传变异主要存在于居群间,遗传多样性与地理关系表现出明显的相关性,ISSR可以作为研究遗传多样性及遗传分化的有效标记。Objective To discuss the genetic diversity of Coptis chinensis. Methods The genetic diversity of 78 individuals from seven populations was analyzed by inter-simple sequence repeat （ISSR）. Results Twelve primers were selected to produce highly reproducible ISSR bands. Among 106 amplified bands, 72 showed polymorphism, the percentage of polymorphic bands reached to 67.92%. Nei＇s gene diversity index （H） was 0. 180 3, Shannon information index （I） was 0. 283 2, Gst was 0. 681 5. The genetic distance coefficient and genetic similarity were 0. 089 4-0. 184 6 and 0. 832 1-0. 912 7, respectively. Conclusion C. chinensis holds high genetic diversity and the majority of genetic variation occurs among the populations. By cluster analysis, the geographical distribution is very obvious. The ISSR marker could be used for the analysis of the genetic diversity and genetic variation of C. chinensis.

关 键 词：黄连 遗传多样性 ISSR 聚类分析 遗传分化 

分 类 号：R282.7[医药卫生—中药学]