福建省沿海地区鼠形动物巴尔通体感染状况调查及基因类型研究  

Study on the prevalence and genotypes of Bartonella species in rodent hosts from Fujian coastal regions

作　　者：叶曦[1] 李国伟[1] 姚美琳[1] 罗炜[1] 苏丽琼[1] 

机构地区：[1]厦门市海沧区疾病预防控制中心,361026

出　　处：《中华流行病学杂志》2009年第10期989-992,共4页Chinese Journal of Epidemiology

基　　金：厦门市医学科技计划项目（WZK-0629） 感谢宁德市蕉城区、福清市、莆田市荔城区、南安市、龙海市疾病预防控制中心参加样本采集的同仁,厦门市疾病预防控制中心何水珍博士对序列分析给予的帮助

摘　　要：目的了解福建省沿海不同地区、不同气温条件下鼠形动物巴尔通体感染状况，并掌握其菌种基因型。方法通过随机抽样，抽取福建省沿海6个地市各一个调查点，采用笼日法捕获鼠形动物，培养分离巴尔通体，疑似菌株通过PCR证实为巴尔通体，并通过gltA基因的379bp片段序列测定，构建生长发育树，确定巴尔通体属种，并分析各属种的地区和宿主分布。结果捕获并培养分离鼠形动物1161只（份），分离到巴尔通体菌株188株，6种被检动物中有5种检出巴尔通体，感染的鼠形动物分属2目2属，分别是臭鼢黯、褐家鼠、黄胸鼠、小家鼠、黑家鼠。鼠形动物总感染率为16．19％，其中臭驹鼯最高（21．43％），其次为褐家鼠（13．54％），黄胸鼠（18．27％）。构成东南地区的主要鼠形动物均列其中。调查6个沿海地市的鼠形动物均感染巴尔通体菌，感染率分别为宁德9．25％、福州9．52％、莆田9．38％、泉州28．18％、厦门17．42％、漳州13．33％。比较年积温7000℃以下地区与年积温7000℃以上地区鼠形动物感染率差异有统计学意义（X^2=12．93，P〈0．001）。序列分析表明福建省沿海地区鼠形动物中共检出3个巴尔通体种群：B．elizabethae、B．qeenslandensis和B．tribocorumA、B群，有明显的地域和宿主分布特征。结论巴尔通体在福建省沿海地区鼠形动物中广泛存在，优势菌种与云南、北京等地区不尽相同。Objective To investigate Bartonella infection in rodent hosts from different environments and types of climate in Fujian coastal regions. Genetypes of the Bartonella strains was also studied to provide scientific basis for prevention and control of the correlated diseases. Methods By random sampling method, we selected six study sites in Fujian southeastern coastal regions. Rodents were captured by cages to Isolate Bartonella strains. Bartonella-like isolates were confirnmed by polymerase chain reaction （PCR）. The 379 bp fragment of gltA gene was sequenced and the growth and development tree was constructed to determine Bartonella species. Distribution of Bartonella species in the different area and related hosts was also analysed. Results Bartonella species were isolated from 188 of 1161 small animals including five rodent species. The infected animals were grouped into 2 genera and 2 orders. They were Suncus murinus, Rattus norvegicus, Rattus flavipectus, Mus musculus and Rattus rattus. The overall prevalence of Bartonella bacteremia was 16.19% in the most prevalent species of rodents in Fujian southeastern coastal regions including 21.43% in Surwus murinus, 13.54% in Rattus norvegicus and 18.27% in Rattus flavipectus. Rodents in every investigated areas were infected by Bartonella species （9.25% in Ningde, 9.52% in Fuzhou, 9.38% in Putian, 28.18% in Quanzhou, 17.42% in Xiamen and 13.33% in Zhangzhou）. There were significant differences among infected rates in different annual accumulated temperature districts （X^2=12.93, P〈0.001）. Isolates from rodents were clustered in three genotypes （B.elizabethae, B.qeenslandensis and B.triboeorum A, B）. Conclusion The local rodents in Fujian southeastern coastal regions were widely infected by Bartonella spp. Differences among the prevalent species of Bartonella in Fujian southeastern coastal region, Yunan and Beijing were noticed. Our findings suggested there was a need to study the prevalence, related vectors and the molecular organism of Bartonella sp

关 键 词：巴尔通体 鼠形动物 分布 基因类型 

分 类 号：S858.9[农业科学—临床兽医学]