中国宿主动物中Amur汉坦病毒M片段全基因序列分析  

Complete sequence of the M segment of Amur virus in rodent from China

作　　者：郭卫东[1] 张文义[2] 江佳富[2] 池海谊[3] 王文瑞[1] 王中元 金鹰 杨红[2] 方立群[2] 曹务春[2] 

机构地区：[1]内蒙古自治区疾病预防控制中心,呼和浩特010031 [2]军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室 [3]呼和浩特市第一医院 [4]黑龙江省韦河林业局卫生防疫站

出　　处：《中华流行病学杂志》2009年第10期1051-1054,共4页Chinese Journal of Epidemiology

基　　金：国家杰出青年基金（30725032）;国家自然科学基金（30590374,30700682）;传染病重大专项基金（2008ZX10004-012）

摘　　要：目的测定中国宿主动物中Amur病毒M片段全基因序列，以了解其分子特征。方法设计特异的PCR引物，用RT-PCR法分段扩增JilinAP06株M片段全长，PCR产物经克隆后进行序列测定，然后进行系统发育分析和重组分析。结果JilinAP06株M片段全基因序列共3615个核苷酸，A＋T含量为59．3％，G＋C含量为40．7％，包含1个单一的开放读码框架，其最大读码框架从41-3448，由3408个核苷酸组成，共编码1135个氨基酸。序列同源分析表明，JilinAP06株与中国的人源Amur HV株同源性为96．0％-97．8％，与韩国大林姬鼠来源的SoochongHV同源性为85．6％．86．7％，与HTNV原型株76—118同源性仅为79．5％，而与其他各型病毒间的同源性均低于79．0％。氨基酸同源分析，其氨基酸序列与中国的人源株Amtll-HV同源性为98．1％～98．4％，与韩国大林姬鼠来源的Soochong HV同源性为96．4％-97．0％，与HTNV原型株76—118同源性为91．7％，而与其他各型病毒间的同源性均低于92．0％。系统发育分析结果显示，JilinAP06与Amur病毒构成一个相对独立的分支。结论从中国宿主动物中获得了AmurHV的M片段全基因序列。Objective To study the complete sequence of M segment of Amur virus in rodents and to explore their molecular characteristics. Methods Complete M segment of Amur virus in rodent from China was amplified by RT-PCR. The purified PCR product was cloned into pGEM-T Easy vector and then sequenced. Phylogenetic analysis on multiple nucleotide sequences was performed with the Tree PUZZLE and DNAStar software. Results The full-length of its M gene comprised of 3615 nucleotides with one open reading frame （ORF） including 3408 nucleotides and encoding a protein which comprised 1135 amino acids. The ORF was located at bases 41 to 3448. The phylogenetic analysis of JilinAP06 with other hantaviruses revealed that the complete sequence of M segment of JilinAP06 strain was closely related to those Amur viruses such as B78 strain, Liu strain and H5 strain were all from the patients. The complete sequence of M segment of JilinAP06 had only 79.5% identities with the nucleotide sequence of HTNV strain 76-118. Conclusion The complete sequence on M segment of Amur virus in rodent was first time identified in this country.

关 键 词：汉坦病毒 Amur病毒 M片段 序列分析 

分 类 号：R3[医药卫生—基础医学]