神经节苷脂的柱前衍生-反相高效液相色谱测定研究  

Determination of gangliosides by reverse phase high-performance liquid chromatography with pre-column derivation

作　　者：孙丽华[1] 李昉赟[1] 王茵[1] 

机构地区：[1]浙江省医学科学院,杭州310013

出　　处：《中国卫生检验杂志》2009年第10期2211-2212,2242,共3页Chinese Journal of Health Laboratory Technology

基　　金：浙江省科技厅分析测试基金项目(2007F70033);浙江省科技厅重大专项基金(2007C12074)

摘　　要：目的:研究建立一种柱前衍生-反相高效液相色谱测定神经节苷脂的分析方法。方法:样品经氯仿-甲醇(2:1)萃取再经对溴苯甲酰甲基溴衍生处理后,用HPLC-DAD分离测定,外标法定量。结果:本方法的检出限:GM1:1.0μg/ml;GM2:2.5μg/ml;GM3:2.0μg/ml。样品回收率>80%,精密度<10%。结论:可为生物组织神经节苷脂分析提供技术参考。Objective：To establish a method for determination of gangliosides by reverse phase high -performance liquid chromatography with pre - column derivation. Methods： The Gangliosides were extracted with chloroform - methanol （ 2 ： 1 ） and then derived with 2,4＇ - dibromo - acetophenone. HPLC separations were conducted using a ZORBAX Eclipse XDB - Cs 250 mm × 4. 6 mm,5μm column, and using methanol - acetonitrile（50 ＋ 50）as the mobile phase and the detection was performed at the wavelength of 260 nm. Results ： The proposed method was accurate, precise and selective with limit of determination of 1.0 μg/ml （GM1）, 2. 5 μg/ml （GM2）, 2.0 μg/ml （GM3）, recovery of more than 80% and RSD of less than 10%. Conclusion： The method can be used for determination of gangliosides in biological tissue.

关 键 词：柱前衍生 反相高效液相色谱 神经节苷脂 

分 类 号：O657.72[理学—分析化学]