流行性腮腺炎病毒微生物检验方法的建立及应用  

A new microbial method for testing mumps virus

作　　者：卜丽薇[1] 周景林[1] 唐一清[1] 

机构地区：[1]北京市通州区疾病预防控制中心,北京101100

出　　处：《中国卫生检验杂志》2009年第10期2322-2323,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:摸索建立流行性腮腺炎(流腮)病毒分离培养和抗体检测方法。方法:从含漱液、尿液标本中分离流行性腮腺炎病毒,从血液标本中检测流行性腮腺炎IgM抗体。结果:共检测样本47件,其中从21件含漱液样本、7件尿液样本中分离出流行性腮腺炎病毒4株,19件血清样本中检测出10件流行性腮腺炎IgM抗体阳性。结论:提高流行性腮腺炎病毒分离和流行性腮腺炎血清IgM抗体阳性检出率,在实验过程中的每一步做好质量控制是非常重要的。Objective：The present study was conducted to establish a new test method for mumps virus isolation, cultivation and serological identification. Methods：Mumps virus was isolated from samples of mouth rinsing water and urine. IgM of mumps was tested from serological samples. Results：Four strains of mumps virus were isolated from 21 samples of mouth rinsing water and 7 samples of urine among all of the 47 samples; 10 samples from 19 blood serums were positive of mumps IgM. Conclusion：The emphasis is to improve the mumps virus isolating level and positive and detective rate of IgM of mumps blood serum and quality control is also important in the process of these tests.

关 键 词：流行性腮腺炎病毒 质量控制 

分 类 号：R446.5[医药卫生—诊断学]