时间分辨免疫荧光法检测乙型肝炎病毒前S1抗原的临床应用  

Clinical application of time-resolved immunofluoresce for detection of hepatitis b virus pre S1 antigen

作　　者：徐爱芳[1] 陈刚[1] 王妙婵[1] 朱秀亚[1] 

机构地区：[1]浙江中医药大学附属杭州市第六人民医院,杭州310014

出　　处：《中国卫生检验杂志》2009年第10期2337-2338,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:采用时间分辨免疫荧光法(TRFIA法)检测乙肝患者前S1抗原,并与酶联免疫吸附法(ELISA法)测定的前S1抗原结果及HBV-DNA测定结果进行比较。方法:分别采用TRFIA法和ELISA法检测362例乙肝患者血清标本的前S1抗原,并进行卡方检验比较。对两种乙肝病毒前S1抗原检测方法结果不符合的标本和部分前S1抗原检测结果在Cutoff值附近的标本进行HBV-DNA的定量检测,并作比较。结果:以ELISA法为参考,TRFIA法的阳性符合率为98.8%,阴性符合率为75.7%,总体符合率为92.0%,两种检测方法的差异有统计学意义(χ2=16.69,P<0.01),TRFIA法的检测阳性率高于ELISA法。与乙肝病毒DNA检测结果相比,TRFIA法差异无统计学意义(χ2=0.25,P>0.05),ELISA法差异有统计学意义(χ2=19.36,P<0.01)。结论:与ELISA法相比,采用TRFIA法检测前S1抗原能更好地反映病人体内乙肝病毒复制情况。Objective： Using time - resolved immunofluorescence assay （TRFIA） to detect hepatitis B patients pre - S1 antigen and comparing the results of enzyme - linked immunosorbent assay （ELISA） and HBV - DNA measured results. Methods： TRFIA method and ELISA method were used separately to detect serum pre - S1 antigen of 362 cases of hepatitis B patients. HBV - DNA was detected to the specimens which PreS1 antigen results did not meet by two methods and some pre - S1 antigen test results are near Cutoff. Results ： Using the ELISA method as a reference, the positive coincidence rate of TRFIA method was 98. 8% and the negative coincidence rate was 75.7%. The overall coincidence rate was 92.0% and the difference between the two methods had statistical significance. The positive rate of TRFIA method was higher than ELISA method. Comprised With hepatitis B virus DNA results. The TRFIA method had no significant difference. And the ELISA method had statistical significance. Conclusion：Compared with the ELISA method, detection of pre S1 antigen with TRFIA method can better reflect the hepatitis B virus replication.

关 键 词：时间分辨免疫荧光法 乙肝病毒前S1抗原 

分 类 号：R446[医药卫生—诊断学]