水通道蛋白1和水通道蛋白3在羊水过少孕妇胎盘和胎膜的表达及其意义  

Expression and significance of aquaporin 1 and aquaporin 3 in placenta and fetal membranes of pregnant women with oligohydramnios

作　　者：朱雪琼[1] 蒋珊珊[1] 王玉环[1] 邹双微[1] 朱雪洁[2] 胡迎春[1] 

机构地区：[1]温州医学院附属第二医院妇产科,325027 [2]温州医学院附属第一医院妇产科

出　　处：《中华围产医学杂志》2009年第5期333-337,共5页Chinese Journal of Perinatal Medicine

基　　金：温州市对外合作交流科技计划项目（H20070042）

摘　　要：目的研究水通道蛋白1（AQP1）和AQP3在羊水过少孕妇胎盘、胎膜中的表达及分布，探讨其在羊水平衡途径中的作用。方法选取30例孤立性羊水过少的足月孕妇为研究组，同期分娩的30例正常羊水量的足月孕妇为对照组。分别采用实时荧光定量PCR和免疫组织化学SP法，检测两组胎盘、胎膜组织中AQP1和AQP3mRNA和蛋白的表达及分布。结果AQP1 mRNA在研究组羊膜组织中的表达为对照组的0．31，AQP1蛋白在研究组羊膜组织中的表达为0．14±0．02，较对照组的0．25±0．03明显下调（P〈0．05）。而在胎盘、绒毛膜中的分布及表达强度两组间差异无统计学意义（P〉0．05）。AQP3 mRNA在研究组羊膜和绒毛膜中的表达分别为对照组的0．31和0．37，而胎盘中的表达为对照组的7．36。AQP3蛋白在研究组羊膜和绒毛膜中的表达分别为0．18±0．05和0．18±0．04，均较对照组的0．26±0．03和0．29±0．06明显下调（P〈0．05），而在胎盘组织中的表达研究组为0．47±0．09，较对照组0．28±0．01明显上调（P〈0．05）。结论AQP1和AQP3在羊水过少孕妇胎盘、胎膜组织中表达的改变为羊水减少后的代偿性反应。Objective To study the expression of aquaporin 1 （AQP1） and aquaporin 3 （AQP3） in fetal membranes and placenta in pregnant women with oligohydramnios and to explore their function in the balance of amniotic fluid. Methods Thirty cases of term pregnancy with oligohydramnios were selected as the experimental group and 30 healthy term pregnant women with normal amniotic fluid volume were served as control. The expressions of mRNA and protein of AQP1 and AQP3 in fetal membranes and placenta were examined by real-time polymerase chain reaction and streptavidi peroxidase immunohistochemiscal staining. Results The expression of AQP1 mRNA in the amnion of the experimental group was 0.31 relative to that of the control group. The expression of AQP1 protein in the amnion of the experimental group was 0.14 ± 0.02, which showed significant decrease when compared with the control （0. 25 ± 0. 03） （P 〈 0. 05）, while no significant difference in the chorion and placenta was found between the two groups（P〉0.05）. The amount of AQP3 mRNA expressions in amnion and chorion in the experimental group were 0.31 and 0.37 relative to those of the control group, respectively, while 7.36 in the placenta. The expression of the AQP3 protein in the amnion and chorion in the experimental group were 0.18±0. 05 and 0.18±0. 04, respectively, which showed significant decrease when compared with those in the control group （0.26 ±0.03 vs 0.29±0. 06, P〈0.05）. But the expression of AQP3 protein in the placenta of experimental group was significantly higher than that of the control （0.47±0. 09 vs 0.28±0. 01, P%0.05）. Conclusions The alterations of AQP1 and AQP3 expressions in fetal membrane and placenta in oligohydramniotic women are adaptive response to oligohydramnios.

关 键 词：水通道蛋白质1 水通道蛋白质3 羊水过少 胎盘 胚外膜 

分 类 号：R714[医药卫生—妇产科学]