以高氯酸·三-2,2′-联吡啶合钴(Ⅲ)作为电子媒介体金纳米颗粒增强的葡萄糖生物传感器  

A highly sensitive glucose biosensor based on immobilization of enzyme on colloidal gold matrix and the complex of cobalt-bipyridyl mediation

作　　者：李群芳[1] 娄方明[1] 周旭美[1] 袁若[2] 

机构地区：[1]遵义医学院药学系,遵义563003 [2]西南大学化学化工学院,重庆400715

出　　处：《分析试验室》2009年第10期17-20,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(20675064);教育部优秀青年资助计划基金(2002-40);遵义医学院硕士科研启动基金(F-305)项目资助

摘　　要：用纳米金溶胶与聚乙烯醇缩丁醛（PVB）构成复合固酶基质,采用溶胶-凝胶法固定葡萄糖氧化酶（GOx）于铂电极表面,并在葡萄糖溶液中加入高氯酸.三-2,2′-联吡啶合钴（Ⅲ）作为电子媒介体,制成了高灵敏的葡萄糖生物传感器。葡萄糖氧化酶吸附在纳米金颗粒表面上稳定且保持其生物活性;而电子媒介体的存在,显著提高了传感器的响应灵敏度。该传感器对葡萄糖响应的线性范围为1.2×10^-8-6.2×10^-6mol/L,检出限6.2×10^-9mol/L（S/N=3）。该生物传感器有效消除了抗坏血酸、尿酸的干扰,可用于人体血清中葡萄糖的测定。A highly sensitive glucose biosensor was fabricated and evaluated in this paper. Glucose oxidase（GOx） adsorbed to colloidal gold sols was combined with polyvinyl butyral（PVB） immobilized onto the surface of a platinum electrode to make a glucose biosensor.The GOx immobilized on colloidal gold was stable and retained its functional activity.With the aid of Co（bpy）33＋ as a mediator,the sensitivity and detection limit（6.25×10^-9 mol/L） of the biosensor for glucose was substantially improved.Moreover,the low operating potential of the sensor can effectively eliminate interference from common interferents, such as ascorbic acid and uric acid, etc.

关 键 词：高氯酸.三-2 2′-联吡啶合钴(Ⅲ) 纳米金 葡萄糖生物传感器 

分 类 号：O657.1[理学—分析化学]