溴酚蓝光度法测定马来酸罗格列酮的含量  

Spectrophotometric method for determination of the content of rosiglitazone maleate with bromophenol blue

作　　者：秦宗会[1] 谭蓉[2] 王杨梅[1] 宁志华[1] 

机构地区：[1]长江师范学院化学及环境科学系,涪陵408100 [2]太极集团涪陵制药厂,涪陵408000

出　　处：《分析试验室》2009年第10期41-44,共4页Chinese Journal of Analysis Laboratory

基　　金：重庆市教委科研(KJ081301);重庆市涪陵区科委科技计划(2008-43);重庆市高等学校优秀中青年骨干教师资助计划[渝教人(2005)2号];长江师范学院学术带头人项目资助

摘　　要：在pH 4.50的Britton-Robinson（B-R）缓冲溶液中,溴酚蓝（BPB）与马来酸罗格列酮（ROM）反应,形成1∶1的离子缔合物,溶液颜色明显发生改变,最大吸收波长为434 nm,同时在波长590 nm处产生负吸收。在590 nm处,马来酸罗格列酮浓度分别在0-1.70×10^-5moL/L范围遵循比尔定律,表观摩尔吸光系数5ε90为4.51×10^4L/（mol.cm）,检出限为2.60×10^-7mol/L。方法可用于分析检测马来酸罗格列酮的含量。In Britton-Robinson（B-R） buffer medium of pH 4.50,bromophenol blue（BPB） can react with rosiglitazone maleate（ROM） to form an ion-association complex of 1∶1,the colours of the solutions change obviously,with its maximum absorption at 434 nm,and negative maximum absorption at 590 nm.Linear relationships were obtained at both maximum absorptions.The concentration of ROM in the range of 0～1.70×10^-5 mol/L obeys beer′s law at negative maximum absorption of 590 nm,the apparent molar absorptivity ε590 is 4.51×10^4 L/（mol·cm）,and the detection limit is 2.60×10^-7 mol/L, the method has applied to the detemaination of rosiglitazone maleate in medicine samples and synthetic samples.

关 键 词：分光光度法 溴酚蓝 马来酸罗格列酮 

分 类 号：O657.3[理学—分析化学]