铌-铬天青S与牛血清白蛋白结合反应及其应用  

Determination of serum protein by chrome azurol S-Nb(Ⅴ) complex spectral probe

作　　者：陶慧林[1] 朱仕毅[1] 梁昊东 

机构地区：[1]桂林工学院材料与化学工程系,桂林541004

出　　处：《分析试验室》2009年第10期53-55,共3页Chinese Journal of Analysis Laboratory

基　　金：广西教育厅科研(桂教科研[2007]34号)项目资助

摘　　要：探讨了铌-铬天青S配合物与牛血清白蛋白（BSA）结合反应体系。在pH 4.6的邻苯二甲酸氢钾-NaOH（Clark-Lubs）缓冲溶液中,在OP存在下,铌-铬天青S配合物与牛血清白蛋白进一步形成复合物,其最大吸收峰位于595 nm,且随着BSA量的增加,体系在595 nm波长处吸收峰也随着增大。据此建立以铌-铬天青S配合物为光谱探针,分光光度法测定蛋白质含量的新方法。牛血清白蛋白含量在0-0.32 mg/mL的范围内符合比尔定律,摩尔吸光系数5ε95为4.4×10^5L.mol^-1.cm^-1,相关系数为γ=0.9996,生物体内的常见物质基本上不干扰测定,方法可用于人血清蛋白中蛋白质的测定。The interaction between chrome azurol S-Nb（Ⅴ） and bovine serum albumin（BSA） was investigated.In a pH 4.6（C-L） buffer solution,with the increase in BSA concentration,the absorption peak at 595 nm increase strikingly.The molar absorptivity is 4.40×105 L·mol-1·cm-1.Beer′s law was obeyed in a range from 0 to 32 mg/mL.A spectrophotometric method for the determination of protein was established by using CAS-Nb（Ⅴ） complex as a spectral probe.The proposed method was applied to the determination of albumin in human serum albumin, and the results were in agreement with certified values. Recoveries of the standard addition for BSA were 95.3% - 106.0% with RSD less than 3.3 % （n = 6）.

关 键 词：铌-铬天青S(CAS)配合物 牛血清白蛋白(BSA) 光谱探针 

分 类 号：O657.3[理学—分析化学]