表面活性剂增敏动力学光度法测定尿液中的微量SCN-  

Kinetic spectrophotometric determination of trace thiocyanate in urine with sensitization effect of surfactant

作　　者：柴红梅[1] 王桂浦[1] 

机构地区：[1]延安大学化学与化工学院,延安716000

出　　处：《分析试验室》2009年第10期75-77,共3页Chinese Journal of Analysis Laboratory

基　　金：陕西省教育厅专项科研计划(08JK489);延安大学创新(D2008-61)项目资助

摘　　要：在pH 11.4的NH3-NH4Cl缓冲溶液中,阳离子表面活性剂溴代十四烷基吡啶（TPB）对硫氰酸根阻抑高碘酸钾氧化间甲酚紫的褪色反应具有明显的增敏作用,据此建立了动力学光度法测定微量SCN-的新方法,该方法的线性范围为0.04-0.72μg/mL,检出限6.74×10^-3μg/mL。可用于测定吸烟和非吸烟者的尿液中的微量SCN^-。A new and simple kinetic spectrophotometric method is proposed for the determination of trace thiocyanate ion.The method is based on the inhibiting effect of thiocyanate on the oxidation of m-cresol purple（m-CP） by KIO4 in a buffer solution of NH3-NH4Cl at pH 11.4.Various surfactants have been studied as enhancing reagents in the system.It was found that the cationic surfactant tetradecyl pyridinium bromide（TPB） could improve the sensitivity and the selectivity apparently.Under optimum experimental conditions, the linear range of this method was 0.04-0.72μg/mL, and the detection limit was 6.74×10^-3 μg/mL. This method has been successfully used for the determination of thiocyanate contents in smokers＇ and non-smokers＇ urine samples.

关 键 词：动力学光度法 硫氰酸根 间甲酚紫 

分 类 号：O657.3[理学—分析化学]