甘附冲剂对维拉帕米致大鼠心动过缓的影响  

Effect of Ganfu Granule on the Verapamil-induced Bradycardia in Rats

作　　者：李东[1] 陈志武[2] 岑德意[2] 邢亚群[3] 王迪生[3] 

机构地区：[1]蚌埠医学院药学系,安徽蚌埠233030 [2]安徽医科大学药理学教研室,安徽合肥230032 [3]安徽蚌埠医学院第二附属医院药剂科,安徽蚌埠233040

出　　处：《辽宁中医药大学学报》2009年第10期174-175,共2页Journal of Liaoning University of Traditional Chinese Medicine

摘　　要：目的:观察甘附冲剂对维拉帕米致大鼠心动过缓的影响,并初步探讨其抗心动过缓可能的作用机制。方法:采用静脉注射(iv)维拉帕米(Ver)制备心动过缓模型,比较ivVer前后大鼠心率的变化,制备心肌匀浆,用Green法测定一氧化氮(NO)的含量,用硫代巴比妥法测定脂质过氧化产物丙二醛(MDA)的含量。结果:甘附冲剂在ivVer后2min对大鼠心率减慢有明显的抑制作用(P<0.05);90、270mg·kg-1甘附冲剂可明显地抑制大鼠心肌组织中NO含量的升高(P<0.05);30、90mg·kg-1甘附冲剂可明显地抑制大鼠心肌组织中MDA含量的降低(P<0.01)。结论:甘附冲剂有明显的抗Ver致心动过缓作用,其抗心动过缓的作用可能与调节心肌组织中NO的含量在适度水平有关。Objective：To observe the effect of Ganfu Granule（Gan） on the verapamil-induced bradycardia in rats and investigate the anti-bradycardic mechanism of Gan. Methods：The bradycardic model in rats was established by verapamil（Ver）.The content of malonaldehyde（MDA） was measured with TBA method, and Green method was used to determine the content of nitric oxide （NO）. Results：Gan obviously inhibited the Ver-induced slowing of heart rate in rats;Gan 90、270mg·kg^-1 obviously reduced the NO content in myocardium;Gan 30、90mg·kg^-1 obviously increased the MDA content in myocardium.Conclusion： Gan has the obvious anti-bradycardic effect via regulating NO content.

关 键 词：心动过缓 维拉帕米 一氧化氮 丙二醛 

分 类 号：R285.5[医药卫生—中药学]