LeERF2基因对旱稻耐盐性的影响  

Effects of LeERF2 Gene to Salt-tolerance of Aerobic Rice

作　　者：常英芬[1] 丁在松[2] 高聚林[1] 

机构地区：[1]内蒙古农业大学农学院,呼和浩特010019 [2]中国农业科学院作物科学研究所,北京100081

出　　处：《生物技术通报》2009年第9期64-68,共5页Biotechnology Bulletin

基　　金：中央级公益性科研院所基本科研业务费专项;国家"十一五"科技支撑计划粮食丰产科技工程项目(2006BAD02A13)

摘　　要：采用T2代转LeERF2基因旱稻(旱297)为材料,研究分蘖期盐胁迫下植株光合性能和生理反应。结果表明,50mM NaCl胁迫条件下,转基因植株(T)和野生型对照(WT)的各项光合参数差异不明显,但在100 mM NaCl胁迫条件下,转基因植株仍能维持较高的光合速率和气孔导度。随着NaCl胁迫浓度增加,野生型旱稻和转基因旱稻植株叶片SOD活性增加幅度加大,但都表现为转基因植株增幅更明显,而MDA含量则表现为野生型植株增幅更明显,表明LeERF2基因增强了旱稻盐胁迫下抗氧化能力,提高了耐盐性,能维持较高光合速率。We now report on the regulatory role of LeERF2 to photosynthetic and antioxidation system in salt response during aerobic rice tillering stage. The results suggested that, after treated by 100 mM NaCl stress , the transgenic aerobic rice maintained higher photosynthesis （Pn） and stomatal conductance （Gs） than wild type. Whereas, after the 50 mM NaCl stress treatment, there was no obvious difference between the wild type and transgenic aerobic rice. The antioxidation system results showed that the transgenic aerobic rice significantly increased SOD activity, and lower level of MDA content than the wild type, further more, the amplitude of variation increases with NaCl stress concentration. It is suggested that the transgenic aerobic rice maintain higher antioxidation ability under salt stress, which is helpful to maintain the higher photosynthesis.

关 键 词：LeERF2基因 旱稻 盐胁迫 响应 

分 类 号：S511.6[农业科学—作物学]