HPLC法测定华盖散传统汤剂与颗粒汤剂中盐酸麻黄碱的含量及体外抑菌作用的比较  

HPLC Determination of Ephedrine and the Comparison of the Effect of Bacteriostasis in HuaGaiSan Traditional Decoction and Kernel Decoction

作　　者：胡占兴[1,2] 靳凤云[3] 武孔云[4] 廖薇[1] 赵杨[3] 梁光义[2,3] 

机构地区：[1]贵州大学,贵州贵阳550025 [2]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550002 [3]贵阳中医学院,贵州贵阳550002 [4]贵阳学院,贵州贵阳550003

出　　处：《中华中医药学刊》2009年第10期2072-2074,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30460154)

摘　　要：目的:测定华盖散传统汤剂、颗粒汤剂中盐酸麻黄碱的含量。方法:采用高效液相色谱法测定华盖散传统汤剂与颗粒汤剂中盐酸麻黄碱的含量,选用Agilent C18柱,以乙腈-0.1%磷酸(4:96)为流动相,1mL/min为流速,207nm为检测波长。采用稀释法体外测定华盖散不同煎液对常见菌种的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。结果:华盖散传统汤剂中盐酸麻黄碱的含量低于颗粒汤剂中的含量,传统汤剂中的平均含量为6.5011mg/处方,颗粒汤剂中的平均含量为7.0901mg/处方。颗粒汤剂对大肠杆菌有抑菌作用而传统汤剂对其没有抑菌作用。结论:该法测定盐酸麻黄碱简便、准确,重复性良好,方中其它成分对测定无干扰。颗粒汤剂比传统汤剂的抑菌作用较强。Objective：Determination of ephedrine in HuaGaiSan traditional decoction and kernel decoction. Methods： HPLC was used. An Agilent SB- C18 eoluran was used, the mobile phase was aeetonitrile-0.1% phosphoric acid （4 ： 96）, the flow rate was lmL/min,the detective wavelength was 207nm. The MIC and MBC of different decoction were determined with the dilution method in vitro. Results ： the content of ephedrine in traditional decoction was lower than that in kernel decoction, the average content was 6. 5011 mg per prescription in traditional decoction and 7.0901 mg per preseription in kernel decoction. Kernel decoction showed the suppressive effect to colon bacillus but traditional decoction did not. Conclusion：The method is simple, convenient, exact and has good reproducibility, the other ingredients have not interferences for the determination,the suppressive effect of kernel decoction is better than that of traditional decoction.

关 键 词：HPLC 盐酸麻黄碱 华盖散 体外抑菌作用 

分 类 号：R285.5[医药卫生—中药学]