寿胎丸抗大鼠同种异体卵巢移植模型急性排斥反应的研究  

Research on the Mechanism of ShouTai Wan Preventing Acute Rejection of Ovarian Allotransplantation in Rats

作　　者：唐丽丽[1] 罗颂平[2] 

机构地区：[1]潍坊市中医院,山东潍坊261041 [2]广州中医药大学,广东广州510405

出　　处：《中华中医药学刊》2009年第10期2098-2101,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：广东省自然科学基金项目(5004236);教育部高等学校博士点基金资助项目(20050572013)

摘　　要：目的:通过观察补肾中药复方寿胎丸对大鼠同种异体卵巢移植模型的影响,来探讨寿胎丸抗卵巢移植急性排斥反应的机理。方法:238只Wistar雌性大鼠随机分为7组:寿胎丸低剂量组;寿胎丸中剂量组;寿胎丸高剂量组;环孢素A(CsA)组;CsA+寿胎丸中剂量组;正常组;模型组。正常组不予手术处理,其余各组均建立同种异体卵巢移植模型,受体Wistar大鼠,供体SD大鼠。观察记录各组动物的体重、活动、皮毛、进食及粪便等一般情况。观察各卵巢移植组大鼠动情周期恢复率。各组于移植术后7、14、21、28、35天分批处死Wistar大鼠,取双侧卵巢,观察移植卵巢生长状况,记录移植卵巢的重量及体积。结果:移植术后,各大鼠动情周期恢复率由高到低依次是:CsA+寿胎丸中剂量组、CsA组、寿胎丸高剂量组、寿胎丸中剂量组、寿胎丸低剂量组、模型组。寿胎丸低、中、高剂量组、CsA组、CsA+寿胎丸中剂量组移植卵巢体积和重量均高于模型组,差异有显著性(P<0.01)。结论:寿胎丸有助于移植术后大鼠动情周期的恢复,与CsA联合应用,协同作用明显。寿胎丸促进移植卵巢的成活,使其重量和体积增加。寿胎丸可能通过以上机理起到抗同种异体卵巢移植模型急性排斥反应的作用。Objective ： To observe the effect and mechanism of ShouTai Wan in treating acute rejection of ovarian allot-ransplantation in SD rats and Wistar rats. Methods ：238 adult female Wistar rats were randomly divided into seven groups： low dose of ShouTaiWan group, middle dose of ShouTaiWan group, high dose of ShouTaiWan group, CsA group, CsA ＋ ShouTaiWan group, normal group, model group. No surgery was performed on the rats in the normal group . Ovarian allot- ransplantation surgery was performed on the rats of other groups. The Wistar rats were killed and evaluated at d7, d14, d21 ,d28 and d35 after transplantation. Generalized case of rats in every group was observed to analyze the side effect of medicines. Rate of rats restoring estrous cycle was observed. Weight and volume of Ovarian grafts were observed. Results ： Rate of rats restoring estrous cycle from high to low was ： CsA ＋ ShouTaiWan group, CsA group, high dose of ShouTaiWan group, middle dose of ShouTaiWan group, low dose of ShouTaiWan group, model group. Weight and volume of Ovarian grafts in low dose of ShouTaiWan group, middle dose of ShouTaiWan group, high dose of ShouTaiWan group, CsA group, and CsA ＋ ShouTaiWan group were significantly higher than those of model group （ P 〈 0.01 ）. Conclusion ： ShouTaiWan helps the recovery of rat estrous cycle, and has obvious synergy combined with CsA. ShouTaiWan raise the survival rate, weight and size of allografting ovaries. ShouTaiWan maybe play the role of preventing acute rejection of allotransplantation in rats through all these mechanisms.

关 键 词：同种异体移植 卵巢 急性排斥反应 寿胎丸 

分 类 号：R285.5[医药卫生—中药学]