化学发光法检测乙型肝炎病毒血清标志物组合模式与分析  

Analysis of Hepatitis B Virus Immunological Marker Models by Chemiluminescent Immunoassay

作　　者：马聪[1] 荣扬[1] 李文伟[1] 刘敏[1] 王海东[1] 吕会净[1] 

机构地区：[1]海军总医院检验科,北京100048

出　　处：《海军总医院学报》2009年第3期139-143,共5页Journal of Naval General Hospital of PLA

摘　　要：目的通过化学发光定量法检测乙型肝炎病毒血清标志物,试图客观地反映乙型肝炎病毒血清标志物的分布状态及其临床意义;并同步对乙型肝炎表面抗原阳性者进行乙型肝炎病毒DNA定量测定,探讨不同乙型肝炎病毒血清标志物与病毒复制的相关性。方法用化学发光定量法和酶联免疫吸附测定法对491例临床样本进行乙型肝炎病毒血清标志物检测及分型。对其中乙型肝炎表面抗原阳性的103例样本进行乙型肝炎病毒DNA测定。结果491例被检者用化学发光法检出15种模式,酶联免疫吸附测定法检出仅9种模式。前者中可见乙型肝炎表面抗原与乙型肝炎抗体双阳(9例,1.84%)和乙型肝炎e抗原与乙型肝炎e抗体双阳(10例,2.03%)等少见模式。检测103例乙型肝炎表面抗原阳性者乙型肝炎病毒DNA阳性63例,占61.16%。乙型肝炎表面抗原和乙型肝炎e抗原测定值在乙型肝炎病毒DNA拷贝数不同分组中差异有统计学意义(P<0.000 1)。结论乙型肝炎病毒血清标志物分布具有多样性;化学发光法的灵敏性和准确性优于酶联免疫吸附测定法,可为临床诊治提供可靠的实验室依据。Objective To analyze the distribution and clinic significance of hepatitis B Virus immunological markers （HBV-M）in serum through measuring the quantitative level of HBV-M by chemiluminescent immunoassay（CLIA）. The relationship between HBV-M and HBV reproduction was investigated through HBV DNA detection in hepatitis B virus surface antigen（HBsAg） positive patients. Methods In sera of 491 patients, HBV-M was detected by both CLIA and ELISA. HBV DNA of 103 specimens with HBsAg positive were measured. Results There were 9 models of HBV-M by ELISA and 6 models by CLIA. Some rare model such as both positive of HBsAg and HBsAb （9 cases, 1.84%）, HBeAg and HBeAb （10 cases,2.03%）were observed by CLIA. In 103 cases with HBsAg positive, there were 63 （61.16%） HBV DNA positive. In samples with different levels of HBV DNA, both HBsAg and HBeAg levels in sera had significant difference（P〈0. 000 1）. Conclusion The results prove the multiformity of HBV-M distribution. The sensitivity and the veracity of CLIA are better than those of ELISA and CLIA can provide reliable data for clinical treatment.

关 键 词：化学发光法 酶联免疫吸附测定法 乙型肝炎病毒血清标志物 乙型肝炎病毒脱氧核糖核酸 

分 类 号：R446.6[医药卫生—诊断学] R373.21[医药卫生—临床医学]