HRSV A_2株细胞低温传代适应减毒指标的初步探索  

STUDY ON THE ATTENUATED EFFECT ABOUT A_2 STRAIN OF HRSV IN CELL CULTURE AT LOW TEMPERATURE

作　　者：谢忠平[1] 龙润乡[1] 李华[1] 易红昆[1] 李志强[1] 沈冬[1] 崔萍芳[1] 陈思瑾[1] 

机构地区：[1]中国医学科学院北京协和医学院医学生物学研究所,昆明650118

出　　处：《现代预防医学》2009年第20期3924-3925,3927,共3页Modern Preventive Medicine

摘　　要：[目的]分析人呼吸道合胞病毒(humanrespiratorysyncytialvirus,HRSV)经人二倍体KMB17细胞低温传代后培养特性的改变。[方法]将HRSVA2株病毒接种到人二倍体KMB17细胞进行低温培养传代,将不同代次的病毒经鼻腔接种豚鼠,于d7、d14分别取肺组织作病理切片检查、分离血清进行中和抗体检测,同时测定不同代次病毒的一步生长曲线及温度敏感性特征。[结果]豚鼠经鼻腔接种,接种原代病毒组d14处死时体重明显较对照组轻,低温第6代组和11代组与对照组接近,3组病毒均引发豚鼠间质性肺炎。低温第6代组和11代组与原代病毒相比,一步生长曲线未发生明显改变,增殖高峰约为9～12d。[结论]HRSV经鼻腔接种豚鼠能引起强烈的肺部感染,豚鼠可作为HRSV毒力评价的动物模型;HRSV在KMB17细胞上经低温传11代,未出现明显的减毒株特征,还需要进一步减毒。[Objective]To analysis the change of human respiratory syncytial virus （HRSV） after generation at low temperature in human diploid cell KMB17. [Methods] The HRSV A2 swains were cultured and passaged in the human diploid cell KMB17 at 32℃lower temperature, the different generation vtrus inoculated to the nose of guinea pigs, and then respectively from lung tissue for biopsy pathology on the 7th days and 14th days, to separate serum for antibody test; To study the growth curve and temperature sensitivity characteristics of the virus. [ Resuitsl The weight of guinea pigs in the group of inoculated primal virus was lighter than the control group on the 14th day. The weight of 6th and 11th group were similar with the-controlgroup. Interstitial pneumonia emerged in the three groups. The step growth curve did not significantly change of the 6th and 11th group virus after passaged at 32℃ lower temperature in cell. and the growth peak was 9-12 days after inoculation. [Gonclu- sion ] HRSV inoculating to the nose of guinea-pig can cause lung infection, guinea-pig can be considered as the animal model of toxicity assessment. The A2 strain was not attenuated on cold-passaged 11th in KMB17 cell at 32℃ lower temperature, still induces interstitial pneumonia.

关 键 词：人呼吸道合胞病毒 低温传代 动物模型 间质性肺炎 生长特性 

分 类 号：R563.1[医药卫生—呼吸系统]