低强度微波对γ射线辐射的防护作用及其可能机制  

Radiation protection and possible mechanisms for low intensity microwave

作　　者：徐茜[1] 童建[1] 靳宗达[1] 陆敏霞[1] 杜厚兵[1] 曹毅[1] 

机构地区：[1]苏州大学放射医学与公共卫生学院卫生毒理学教研室,江苏省放射医学与防护重点实验室,215123

出　　处：《中华劳动卫生职业病杂志》2009年第9期520-524,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：江苏省高校自然科学基金重大项目（05KJA33013）;江苏省放射医学与防护省级重点实验室开放课题（KJS05027）

摘　　要：目的研究低强度微波对受γ射线照射小鼠的辐射防护作用及其可能机制。方法96只雄性昆明种小鼠随机分为对照组、微波照射组（微波组，120μW／cm^2,900MHz，比吸收率为0．4W．／kg）、电离组（5Gyγ射线照射）及微波与γ射线复合照射组（复合组，120μW／cm。微波＋5Cy γ射线照射），每组24只。微波组与复合组小鼠先接受功率密度为120μW／cm^2的微波照射，1h／d，持续14d，第15天给予电离组和复合组5Gy^60Co γ射线一次性全身照射。于照射后3、6、9及12d处死动物，固定胸骨及脾脏，作病理观察，流式细胞仪检测受照射小鼠的脾细胞凋亡率，同时测定血清中抗氧化酶和脂质过氧化物水平。结果骨髓病理切片显示，单独γ射线与复合照射均可导致骨髓组织经历典型的凋亡坏死、空虚、再生修复和恢复4个阶段的病理改变，但复合组的病变轻于电离组，恢复也更快；脾损伤病变过程与骨髓造血组织基本相似，复合组病变轻于电离组。流式细胞仪结果显示，照后6、9d复合组脾细胞凋亡率（分别为23．02％±15．18％、25．37％±11．62％）明显低于电离组，差异有统计学意义（p〈0．05）；氧化损伤检测表明，复合组照后3、9、12d血清中超氧化物歧化酶（SOD）活力较电离组明显升高，6、9、12d丙二醛（MDA）含量明显降低，差异均有统计学意义（P〈0．05）。结论特定强度的微波辐射具有一定的辐射防护作用，其作用机制可能与抑制辐射诱导的造血细胞凋亡和抗氧化损伤作用有关。Objective To investigate radiation protection and possible mechanisms of low intensity microwave on γ-ray exposed mice. Methods 96 healthy Kunming mice were randomly divided into the following four groups： normal control, microwave （120μW/cm^2, 900 MHz）, γ-ray irradiation （5 Gy）, combined exposure of microwave and γ-ray（120 μW/cm^2＋5 Gy）. The microwave group and combined group were exposed to 120 μW/cm^2 microwave firstly, 1 h/d, for 14 days. Then the ionization and combined group were exposed to 5 Gy ^60Co γ-ray irradiation on the 15th day. Animals were sacrificed on the third ,6th ,9th and 12th day after irradiation. The sternum and spleen paraffin section were produced, and the histological changes were observed. Apoptosis rate of mice splenic cells in each group was examined by flow cytometry, and serum concentration of antioxidase and lipid peroxide was detected at the same time. Results Bone marrow was obviously injured either by radiation or microwave exposure, characterized by undergoing four-phase lesions, namely apoptosisnecrosis, void, regeneration and recovery phase. Compared with the T-ray group, the pathological changes in combined group were slighter and the recovery was quicker. The pathological injuries of spleen were similar to that of bone marrow. Injuries in the combined group were slighter than T-ray group. It showed that apoptosis rate of splenic ceils in combined group was significantly lower on the 6th and 9th day after γ-ray radiation （23.02%±15.18%,25.37%±11.62% respectively） from FCM results. Assays of oxidative damages suggested that serum superoxide dismutase（SOD）level in combined group increased while lipid peroxide level decreased significantly（P〈0.05 ）. Conclusion Low intensity microwave may exert protection effects on injuries induced by ionizing radiation. The underlying mechanisms might be related with suppression on the hematopoietic ceils apoptosis induced by γ-ray radiation, inhibition of oxidative damages, and thus enhanced reconstruc

关 键 词：微波 Γ射线 辐射防护 

分 类 号：R686[医药卫生—骨科学]