检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:邱联波[1] 丁桂荣[1] 张亚美[2] 周艳[2] 王晓武[1] 李康樗[1] 徐胜龙[1] 谭娟[1] 周家兴[1] 郭国祯[1]
机构地区:[1]第四军医大学放射医学教研室,西安710032 [2]电子科技大学生命科学学院
出 处:《中华劳动卫生职业病杂志》2009年第9期539-543,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases
基 金:国家自然科学基金资助项目(30670492);全国优秀博士学位论文作者专项基金(200465);国家高技术研究发展计划(863)(2006AA0224C3)
摘 要:目的研究电磁脉冲(electromagnetic pulse,EMP)辐照对大鼠血脑屏障通透性及血脑屏障紧密连接(tight junction,TJ)相关蛋白的影响。方法66只健康成年雄性SD大鼠按体重随机分为假辐照组和辐照组,EMP辐照参数为:场强200kV/m,脉宽7ns,脉冲次数200,脉冲重复频率1Hz。辐照后不同时间取材,用白蛋白免疫组织化学方法观察EMP辐照后血脑屏障通透性的变化,免疫印迹法检测脑组织和脑微血管中TJ蛋白(occludin和ZO—1)的表达情况,免疫荧光方法观察微血管紧密连接蛋白的分布情况。结果免疫组织化学结果显示,辐照后0.5h即可见有白蛋白漏出的微血管,但数目较少,随辐照后时间延长,有白蛋白漏出的微血管数目逐渐增加,至辐照后3h达到高峰,辐照后6h时开始恢复,辐照后12h基本恢复至对照水平;免疫印迹结果显示,occludin蛋白水平与对照组相比无明显差异;ZO-1蛋白水平在辐照后1、3h明显降低,并且免疫荧光结果显示ZO-1在微血管上的分布亦发生了改变,由辐照前的连续分布变为断续分布。结论EMP辐照后ZO-1蛋白水平和分布的改变可能是EMP致大鼠血脑屏障通透性增加的分子机制之一。Objective To study the effect of electromagnetic pulse(EMP) on the permeability of blood-brain barrier, tight junction (TJ)-associated protein expression and localization in rats. Methods 66 male SD rats, weighing (200-250) g, were sham or whole-body exposed to EMP at 200 kV/m for 200 pulses. The repetition rate was 1 Hz. The permeability of the blood-brain barrier in rats was assessed by albumin immunohistochemistry. The expression of typical tight junction protein ZO-1 and occludin in both cerebral cortex homogenate and cerebral cortex microvessel homogenate was analyzed by the Western blotting and the distribution of ZO-1 and occludin was examined by immunofluorescence microscopy. Results In the sham exposure rats, no brain capillaries showed albumin leakage, at 0.5 h after 200 kV/m EMP exposure for 200 pulses; a few brain capillaries with extravasated serum albumin was found, with the time extended, the number of brain capillaries with extravasated serum albumin increased, and reached the peak at 3 h, then began to recover at 6 h. In addition, no change in the distribution of the occludin was found after EMP exposure. Total occludin expression had no significant change compared with the control. However, the expression level of ZO-1 significantly decreased at 1 h and 3 h after EMP exposure in both cerebral cortex homogenate and cerebral cortex microvessel homogenate. Furthermore, immunofluorescence studies also showed alterations in ZO-1 protein localization in cerebral cortex microvessel. Conclusion The EMP exposure (200 kV/m, 200 pulses) could increase blood-brain barrier permeability in rat, and this change is associated with specific alterations in tight junction protein ZO-1.
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:3.142.243.141