新型鸭肝炎病毒的分离鉴定  

Isolation and characterization of a new serotype of duck hepatitis virus

作　　者：范书才[1] 李虹[1] 袁率珍[1] 巢伟 王少英 林旭野 张毓金 朱山林 张兵[1] 姜畔[1] 范小舟[3] 

机构地区：[1]中国兽医药品监察所,北京100081 [2]广东永顺生物制药有限公司,广东广州511356 [3]四川大学华西公共卫生学院,四川成都610041

出　　处：《中国预防兽医学报》2009年第10期770-775,共6页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家科技基础条件平台建设项目(2005DKA21205)

摘　　要：对从广东地区流行鸭肝炎病例中分离到的1株病毒(命名为"GD株")进行了鸭胚(DE)传代培养。DE2～DE6代鸭胚毒的ELD50在10-5.17/0.2mL～10-6.38/0.2mL之间。DE6代毒回归雏鸭后可以产生与1型鸭肝炎病毒(DHV)相同的临床症状和解剖病变。鸭肝组织毒对4日龄、8日龄和16日龄雏鸭的LD50分别为10-6.5/0.5mL、10-5.38/0.5mL和10-4.83/0.5mL。病毒对氯仿、乙醚和胰酶不敏感,能够耐受pH3.0酸处理,62℃30min不能被完全灭活。病毒核酸类型鉴定为RNA病毒。生物学和理化学特性鉴定结果表明,GD株为强毒DHV。使用标准1型DHV阳性血清进行的交叉中和试验和交叉被动保护试验表明,GD株病毒在抗原性上与1型DHV无关。对DHV抗原相关的VP1基因序列分析和同源性比较表明,GD株与DHV-1的VP1核苷酸序列同源性为69.82%,氨基酸序列同源性为71.77%。GD株属于一种在我国流行的新型DHV强毒(暂命名为"CN-DHV")。A virus strain （designated as GD strain ） was isolated from dead ducklings with duck hepatitis in Guangdong province. The virus could cause death over 80 % in 10-11 day-old duck embryos when inocubatied with 100 ELD50 per 0.2 mL. Intramuscular injection of duck embryos viruses to ducklings caused same clinical symptoms and pathological changes as the type 1 duck hepatitis virus （DHV-1）. The virus was not sensitive to chloroform, diethyl ether and trypsin and possessed acid-resistant and heat-resistant features. The GD strain belongs to a virulent DHV based on biological and physicochemical characteristics, but was not related to DHV-1 as shown by in vitro cross-neutralization assay and in vivo cross-protection test with DHV-1 antiserum. Sequence comparison showed that the nt and aa sequences of VP1 of GD strain and DHV-1 shared identities of 69.82 % and 71.77 %, respec- tively. Therefore, GD strain represents a new serotype of DHV occurred in China （designated CN-DHV）. Key words： duck hepatitis virus （DHV）; new serotype of DHV; isolation and identification

关 键 词：鸭肝炎病毒 新型鸭肝炎病毒 分离鉴定 

分 类 号：S852.65[农业科学—基础兽医学]