大鼠自体肝移植后胰腺损伤的研究  

Research on the pancreatic injury after autologous liver transplantation in rats

作　　者：李勇[1] 张培建[1] 郎洁[1] 冯敏[1] 金成[1] 周斌[1] 陶立德[1] 刘新颜[1] 

机构地区：[1]扬州大学第二临床医学院普通外科研究室,225001

出　　处：《中华消化外科杂志》2009年第5期364-366,共3页Chinese Journal of Digestive Surgery

基　　金：江苏省卫生厅医学科研招标立项课题（Z200515,H200770）

摘　　要：目的探讨大鼠自体肝移植后胰腺损伤的原因。方法42只SD大鼠按随机数字表法分为自体肝移植1、6、12、24、48、72h组和假手术组（每组各6只）。假手术组在术后立即取样，自体肝移植各组分别在术后1、6、12、24、48、72h取样。测定血清淀粉酶、脂肪酶，了解胰腺外分泌功能；光镜、电镜观察胰腺组织形态学改变情况。采用单因素方差分析检验结果。结果自体肝移植1h组的大鼠血清淀粉酶、脂肪酶含量高于假手术组，随着术后时间的延长，其含量逐渐增加，自体肝移植48h组的大鼠血清淀粉酶、脂肪酶含量达到高峰，随后下降。各组比较差异有统计学意义（F=538．622，489．417，P〈0．05）。光镜下，自体肝移植1h组大鼠胰腺组织有水肿型胰腺炎的表现，自体肝移植6h组大鼠胰腺组织有出血坏死型胰腺炎的表现，自体肝移植48h组大鼠胰腺组织达到损伤高峰。电镜下，自体肝移植1h组大鼠胰腺细胞出现线粒体增多、肿胀，内质网、高尔基体肿胀，线粒体的面积、周长、比表面、线粒体平均灰度值分别为（312±40）mm^2、（80．3±3．8）mm、0．332±0．039、113±11。随着时间延长，损伤逐渐加重，出现自噬体。自体肝移植48h组大鼠胰腺细胞达到损伤高峰，其线粒体的面积、周长、比表面、平均灰度值分别达到（466±7）mm^2、（108．8±3．7）mm、0．298±0．009、195±12。各组线粒体比表面和平均灰度值比较差异有统计学意义（F=9．322，76．560，P〈0．05）。结论大鼠自体肝移植后胰腺损伤与缺氧引起细胞能量代谢有关。Objective To investigate the causes of pancreatic injury after autologous liver transplantation in rats. Methods Forty-two SD rats were randomly divided into post autologous liver transplantation 1-hour group, 6-hour group, 12-hour group, 24-hour group, 48-hour group, 72-hour group and sham group （6 rats per group）. The plasma concentrations of amylase and lipase were measured to assess pancreatic exocrine function. The histomorphological changes of pancreatic tissue were studied under optical and electron microscopes. All data were analyzed via one-way ANOVA. Results The plasma concentrations of amylase and lipase in post autologous liver transplantation 1-hour group were significantly higher than those in sham group, and they gradually increased as time passed by. The plasma concentrations of amylase and lipase reached peak at hour 48, after which they decreased gradually. There was a significant difference in the plasma concentration of amylase and lipase among the 7 groups （F = 538. 622,489.417, P 〈 0.05 ）. Acute edematous pancreatitis was observed 1 hour after autologous liver transplantation, and acute hemorrhagic necrotic pancreatitis was observed 6 hours after transplantation. The degree of injury reached a peak 48 hours after transplantation. The number of mitoehondria was increased, and endoplasmic reticulum and Golgi apparatus were swollen 1 hour after transplantation, and the area, perimeter, specific surface area and mean gray value of mitochondria were （312 -± 40 ）mm2, （80.3 ± 3.8 ）ram, 0. 332 ± 0.039 and 113 ± 11, respectively. As time passed by, the injury of the pancreatic cells was aggravated and autophagosomes were observed. The injury was most severe 48 hours after transplantation, and the area, perimeter, specific surface area and mean gray value of mitochondria were （ 466 ± 7 ） mm^2, （ 108.8 ± 3.7 ） mm, 0. 298 ± 0.009 and 195 ± 12, respectively. There were significant differences in the specific surface area and mean gray value among all the groups （ F = 9

关 键 词：肝移植 胰腺 损伤 形态学 大鼠 

分 类 号：R657.3[医药卫生—外科学] R657.5[医药卫生—临床医学]