外源多胺对莴苣种子萌发诱导及其与一氧化氮的关系  

Relationship between Exogenous Polyamines and Nitric Oxide and Their Induced Effects on Lettuce Seed Germination

作　　者：白旭[1] 苏国兴[1] 孙娜[1] 王立伟[1] 顾福根[1] 

机构地区：[1]苏州大学基础医学与生命科学学院,江苏苏州215123

出　　处：《西北植物学报》2009年第9期1860-1866,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：苏州大学博士启动基金(13120740)

摘　　要：以正常莴苣‘挂丝红’(Lactuca sativa)种子为材料,采用外源多胺(Put、Spd、Spm)、硝普钠(SNP)及NO清除剂cPTIO处理,以DAQ作为NO荧光检测探针,研究多胺和NO在莴苣种子萌发过程中的相互关系。结果显示:(1)外源多胺尤其是亚精胺(Spd)对莴苣种子都有一定的促早萌作用并以0.5 mmol.L-1处理最佳,其效果在种子吸胀后前48 h最为显著;1.0 mmol.L-1亚精胺合酶抑制剂环己胺(CHA)对莴苣种子萌发有较强的抑制作用;(2)外源NO供体SNP对莴苣种子早萌有显著的促进作用并表现出浓度依赖性,且48 h后促进作用消失,与外源Spd促进种子早萌作用相似;(3)在外源Spd和SNP处理的同时,添加NO清除剂cPTIO可显著降低SNP和Spd对莴苣种子萌发的促进作用;在Spd和SNP处理后的种子近胚区均有较强的NO荧光产生,而cPTIO可猝灭Spd和SNP处理种子胚荧光的增强,并伴随着对Spd和SNP促进种子早萌作用的抑制。研究表明,多胺尤其是亚精胺在促进莴苣种子早萌过程中可能与NO的诱导产生有关。Normal lettuce seeds（Lactuca sativa L.cv Guasihong） were used to understand the relationship between polyamines and nitric oxide（NO） in the process of seed germination after treatments with exogenous polyamines and NO donor SNP（sodium nitroprusside） together with NO specific scavenger cPTIO.The results showed that exogenous polyamines,especially Spd,had a certain role in promoting early germination and the optimal concentration was 0.5 mmol·L^-1.Their effect was more significant before 48 h after seed imbibition.Consistent with the result,1.0 mmol·L^-1 cyclohexylamine（CHA,spermidine synthase inhibitor） had a strong inhibitory effect on the germination of lettuce seeds.Like Spd,exogenous NO donor SNP also promoted seed early germination of lettuce,but in a concentration-dependent way,its function disappeared after 48 h.When treated with SNP and Spd,application of NO scavenger cPTIO could markedly reduce the SNP-or Spd-promoted effect on lettuce seed germination.Fluorescent detection using the DAQ,a NO-sensitive fluorescent probe,showed that there was strong NO fluorescence near seed embryo treated with Spd or SNP.The fluorescence could be eliminated by cPTIO concomitantly with its inhibitory effect on the early germination promoted by Spd or SNP.In this paper,we preliminarily approve that polyamines-,especially spermidine-induced early germination of lettuce seeds may be relevant to the NO induction.

关 键 词：莴苣 亚精胺 一氧化氮 种子萌发 

分 类 号：Q945.78[生物学—植物学]