大鼠外周神经损伤后许旺细胞表型改变的初步探讨  

Phenotypic changes of Schwann cells after sciatic nerve injury of rats in vivo

作　　者：王瑾[1] 王艳华[1] 张培训[1] 寇玉辉[1] 张宏波[1] 姜保国[1] 

机构地区：[1]北京大学人民医院创伤骨科北京大学交通医学中心,100044

出　　处：《中华显微外科杂志》2009年第5期378-380,I0004,共4页Chinese Journal of Microsurgery

基　　金：国家自然科学基金杰出青年基金（30625036）.国家自然科学基金（30801169）

摘　　要：目的探讨用免疫标记方法观察外周神经修复过程中许旺细胞表型改变的意义。方法用SD雄性大鼠18只，制作右侧坐骨神经损伤模型，模拟临床常见的神经外膜相对扭转的缝合方法，分别于术后1、2、3周取缝合点远近各2mm长坐骨神经标本，采用GFAP、Sox2、Krox20抗体标记许旺细胞。结果术后各观察点许旺细胞的GFAP表达均高于正常，且1周时表达最为明显；未见Sox2表达；术后1周Krox20几乎不表达，2、3周时Krox20的表达均高于正常，且Krox20阳性的许旺细胞明显增殖。结论通过免疫标记方法可以反映修复不同阶段许旺细胞的分化状态；对此现象的观察有利于判断神经再生状态并寻找影响神经再生的因素。Objective To describe the rule of the phenotypic changes of schwann cells after peripheral nerve injury. Methods All experiments were performed on 18 male SD rats. Right midseiatic nerves were cut and were repaired by epineurium suture. At selected time points after injury（lweek,2 weeks, 3weeks; n = 6 rats for each time point）, the proximal and distal sciatic nerve stumps （approximately a 2 mm length of nerve to the injured tip） were removed for detecting the expressions of GFAP, Sox2 and Krox20 by immunofluorescence methods. Results After sciatic nerve transection, there was expansion in the population of GFAP-labeled Schwann cells both in the proximal and distal stumps, and those expression reached the peak near to 7 d. Sox2 was neural stem cell markers and there was no Sox2-expressed cells stumps after nerve injury. And Krox20 positive Schwann cells continuously decreased in the first week, then had increased. Conclusion The differentiated stage of Schwann cells during peripheral nerve repair can be detected by using immunolabeling, and the observation above may be beneficial to find methods in improving nerve regeneration.

关 键 词：外周神经 坐骨神经 许旺细胞 表型 

分 类 号：R651.3[医药卫生—外科学] R589.2[医药卫生—临床医学]