人脐血间充质干细胞修复大鼠坐骨神经损伤的实验研究  

Experiment research of the function of human umbilical cord blood mesenchymal stem cells in the regeneration of rat＇s sciatic nerve

作　　者：王忠仁[1] 杨波[2] 王利民[3] 李恩[2] 

机构地区：[1]河南省焦作市人民医院骨科,454002 [2]郑州大学第一附属医院神经外科 [3]郑州大学第一附属医院骨科

出　　处：《中华显微外科杂志》2009年第5期387-389,I0006,共4页Chinese Journal of Microsurgery

摘　　要：目的评价用人脐血间充质干细胞（HUCBMSCs）构建组织工程化人工神经修复大鼠坐骨神经10mm缺损的治疗效果。方法用淋巴细胞分离液分离脐血的单个核细胞．以偏酸性的Mesencult^TM进行培养获得MSCs。30只SD大鼠随机分为3组，每组10只。A组：将HUCBMSCs与生物蛋白胶混合，种植于羊膜管中修复坐骨神经缺损；B组：仅将生物蛋白胶种植于羊膜管中：C组：坐骨神经切下后再将其缝合。9周后，行大体观察、坐骨神经功能指数、腓肠肌湿重测定、组织学染色，S100免疫组化染色等检查。结果32份脐血18份可培养出MSCs，但传代培养大量扩增只有4份。HUCBMSCs植入手术后9周检查结果显示．坐骨神经功能指数、腓肠肌湿重测定A组（-64．2234±2．9461、41．29524±3．88421）优于B组（-74．5882±3．3298、28．34097±3．42889），差异有统计学意义（P〈0．05）。结论人脐血中含有MSCs并且能够分离培养扩增，HUCBMSCs能够促进大鼠坐骨神经损伤的神经再生。Objective To evaluate the efectiveness of using mesenchymal stem cells （MSCs） derived from HUCB（human umbilical cord blood） to a tissue engineered bioartificial nerve on bridging a 10 mm sciatic nerve gap. Methods The cord blood mononuclear cells were isolated by lymphocyte separation medium, purified and expanded with MesencultTM medium and acidic environment to produce adherent layer （MSCs）. Thirty SD female rats were randomly divided into three groups. Group A： Human amnion tubes were seeded by HUCBMSCs together with fibrin sealant. Group B： Human amnion tubes were seeded only with fibrin sealant. group C： autografts. 9 weeks later, a series of examinations were performed which included morphological, sciatic nerve function index, weight of gastrocnemius, histological staining and immunostaining of SIO0. Results The HUCB-derived mononuclear cells, when seeded in specific medium, gave rise to adherent cells （MSCs）. At 9 weeks after the operations, all the examinations of group A was better than group B（P 〈 0.05）. Conclusion HUCBMSCs can be isolated, purified, cultivated and expanded MesencultTM medium. HUCBMSCs can promote the nerve to regenerate in reparing the sciatic nerve gap.

关 键 词：脐血 间充质干细胞 坐骨神经 神经再生 组织工程 

分 类 号：R651.3[医药卫生—外科学]