银杏叶提取物对实验性糖尿病大鼠肾组织HGF/c-met表达的影响  

Effect of Ginkgo Biloba Extract(EGb) on Renal Expression of HGF/c-met in Diabetic Rats

作　　者：孙健[1] 李英[1] 腊岩[1] 高岩[1] 杨青[1] 卞鲁岩[1] 蔡琰[1] 

机构地区：[1]山东省青岛市市立医院肾内科,青岛266071

出　　处：《中国中西医结合肾病杂志》2009年第10期877-881,I0004,共6页Chinese Journal of Integrated Traditional and Western Nephrology

摘　　要：目的:研究银杏叶提取物(ginkgo biloba extract,EGb)对实验性糖尿病大鼠肾脏保护作用及其可能机制。方法:SD大鼠随机分为糖尿病对照组(DC)、银杏叶提取物(金纳多)治疗组(DG)和正常对照组(NC),观察各组大鼠肾脏病理改变;应用免疫组化监测HGF、TGF-β1表达量,运用RT-PCR监测HGF、c-met的mRNA表达情况。结果:免疫组化显示:HGF在NC组微量表达于肾小球系膜区,DC组第2周表达明显增加,与NC组相比差异有统计学意义(P<0.01),第6周表达较前明显下降;DG组第2周表达明显增加,第6周表达较前有所下降,与NC组同期相比差异有统计学意义(P<0.01),与DC组相比差异有统计学意义(P<0.05);TGF-β1在NC组中无阳性表达,DC组表达明显升高,与NC组同期相比差异有统计学意义(P<0.01),DG组表达升高,但低于DC组(P<0.01),与NC组同期相比差异有统计学意义(P<0.01);RT-PCR结果显示:HGF/c-metmRNA在NC组中有一定量表达,第2周与第6周之间差异无统计学意义(P>0.05);DC组与NC组相比,在第2周表达量升高(P<0.01),第6周表达量较前明显下降;DG组与DC组平行相比表达量显著升高(P<0.01)。结论:HGF在DN的病程进展中发挥着重要作用,EGb可以抑制ACE,下调TGF-β1的表达,上调HGF/c-met的表达,减轻肾小球系膜区增生,改善肾功能。Objective： To investigate the protective effect of Gin - kgo biloba extract and its mechanism in diabetic rats. Methods： Sprague - dawley（SD） rats were randomly divided into 3 groups： normal control（ NC）, diabetic control （DC） and diabetic rats treated with EGb（DG）. Renal tissues were examined routinely by light microscope and electron. HGFand TGF-β1 were measured by histoimmunochemical methods. The mRNA of HGF and c - met in renal were measured by RT - PCR. Results： By histoim- munochemical methods HGF was microscale expressed on glomerular mesentery of group NC （P〈 0.01 ）. That was more expressed in group DC and DG than group NC,but did to fall down 6th week;coversly the expression of TGF- β1 in group DC and DG, was more and more significantly higher than that in group NC（ P 〈 0.01 ）. The difference between group DC and IX.; was significantly （P〈0.01）. By RT- PCR the exprexsion of HGF and c- met mRNA were higher in diabetic groups after 2weeks than the normal （ P 〈 0.01 ）, then fell down 6th week, That was significantly higher in group DG than group DC （ P 〈 0. 01 ）. Conclusion： HGF plays an important role in the onset and progression of DN. EGb can suppress ACE, reduce expression of TGF-β1, increase that of HGF/ c- met, to suppress glomerularmesangial matrix hyperplasia and meliorate the real function.

关 键 词：糖尿病肾病 银杏叶提取物 肝细胞生长因子 转化生长因子-β1 肝细胞生长因子受体 

分 类 号：R285.5[医药卫生—中药学]