^11C-胆碱和^18F—FDG PET反映兔VX2肺肿瘤增殖活性的对比研究  

Correlation between ^11 C-choline or ^18F-FDG uptake and tumor proliferation： a rabbit bearing lung cancer model study

作　　者：李亚军[1] 白人驹[1] 高硕[1] 李彦生[1] 刘磊[1] 贾蔚[1] 蔡莉[1] 邢喜玲[1] 

机构地区：[1]天津医科大学总医院PET/CT中心,300052

出　　处：《中华核医学杂志》2009年第5期306-310,共5页Chinese Journal of Nuclear Medicine

摘　　要：目的探讨^11C-胆碱和^18F-脱氧葡萄糖（FDG）PET反映实验性兔VX2肺肿瘤细胞增殖活性的价值。方法新西兰大白兔60只，6只用于传代，另54只于全身麻醉状态下，采用经皮穿刺法于右肺注射VX2肿瘤活细胞悬液0．5ml，10～11d后行PET检查。胆碱PET于静脉注射37MBq^11C-胆碱5rain后进行，^18F—FDG PET于静脉注射18．5MBq^18F—FDG后60min、^11C-胆碱注射后120min进行。测量肿瘤最大标准摄取值（SUVmax）。HE染色观察肿瘤细胞密度，增殖细胞核抗原（PCNA）免疫组织化学染色评价肿瘤细胞增殖活性。^11C-胆碱SUVmax和^18F—FDG SUVmax的比较采用配对t检验，SUVmax与肿瘤细胞密度及PCNA指数之间进行Pearson直线相关分析。结果33只实验兔成功种植VX2肿瘤，并完成所有影像学检查。肿瘤的^11C-胆碱SUVmax为4．02±3．07（1．4～12．2），^18F-FDG SUVmax为5．70±3．45（1．0～13．0），后者明显高于前者（t=-3．188，P：0．003），二者呈正相关（r=0．578，P〈0．05）。高倍光学显微镜下（×200，0．739mm^2视野），肿瘤细胞密度为（547．36±64．78，413～708）个，PCNA指数为（42．34±15．26）％（3．23％-75．87％）。^11C-胆碱SUVmax与PCNA指数呈正相关（r=0.786，P〈0．05），r^2=0．617，但与肿瘤细胞密度之间不具有相关性（r=-0．176，P=0．327）。FDG SUVmax与PCNA指数呈正相关（r=0．551，P=0．001），r^2=0．281，与肿瘤细胞密度之间不具有相关性（r=-0．105，P=0．561）。结论^11C-胆碱与^18F—FDG PET均可反映肿瘤细胞增殖活性，以^11C-胆碱PET的准确性更高。Objective Tumor proliferative activity has been recognized as an indicator of malignant degree in lung cancer and related to prognosis. The purpose of this study was to evaluate the feasibility of assessing proliferative activity with ^11 C-choline and ^18F-fluorodeoxyglucose （FDG） PET on a rabbit bearing lung VX2 tumor model. Methods About 0.5 ml of viable VX2 tumor cell suspension was slowly injected into the right lungs of 54 New Zealand white rabbits through a transthoracical needle insertion. ^1l C-choline and ^18F-FDG PET scan were performed 10- 11 d after tumor implantation. One ear vein was cannulated for administration of the tracers. ^11 C-choline PET scan （with Discovery LS PET/CT scanner, GE ） was performed 5 rain after intravenously injection of 37 MBq ^11 C-choline. Then 18.7 MBq ^18F-FDG was infused at 60 rain after ^11 C-choline administration and ^18 F-FDG PET scan was performed at 60 min after ^18 F-FDG administration. The maximal standardized uptake value of tumor was calculated. The animals were euthanized after examination. Histochemical stain with proliferating cell nuclear antigen （ PCNA ） was performed and PCNA index was obtained to assess tumor proliferation. The difference of ^11 C-choline and ^18F-FDG was analyzed using paired student t-test. The correlation of ^11 C-choline ^18F-FDG and tumor cell density and PCNA index was analyzed using Pearson linear regression. Results Of the 54 rabbits, 36 had a solitary pulmonary tumor. The rate of successful generation of a solitary VX2 tumor was 66.7% （36/54）. Only 33 rabbits underwent both ^11 C-choline and ^18F-FDG PET, and enrolled in this study. The mean cellular density was （547.36± 64.78） cells/field and the mean PCNA index was （42.34 ± 15.26）%. ^18F-FDG was higher than ^11 C-choline （5.70 ± 3.45 vs 4.02 ± 3.07, t = - 3. 188, P = 0. 003 ）. ^11 C-choline significantly and positively correlated with PCNA index （ r = 0. 786, P 〈 0.05 ）. However, there was no significant correla- tion between ^11 C-cholin

关 键 词：肺肿瘤 体层摄影术 发射型计算机 胆碱 脱氧葡萄糖 兔 

分 类 号：R686[医药卫生—骨科学]