采用近红外透射光谱对紫杉醇注射液中紫杉醇含量的快速测定  

Determination of Taxol in Taxol Injection Using Near Infrared Transmission Spectroscopy

作　　者：徐珽[1] 周旻[2] 王磊磊[2] 叶利明[2] 陈聪[2] 唐尧[1] 黄果 吴严巍 

机构地区：[1]四川大学华西医院药剂科,成都610041 [2]四川大学华西药学院,成都610041 [3]布鲁克光谱仪器公司,北京100081

出　　处：《生物医学工程学杂志》2009年第5期982-984,共3页Journal of Biomedical Engineering

基　　金：国家"十五"科技攻关项目基金资助(2001BA701A11)

摘　　要：提出采用近红外光谱技术(Near infrared spectra,NIR)快速测定抗癌药紫杉醇注射液中紫杉醇含量的方法,实现医院药房对注射液使用前的再测定。对24个月内生产的不同批次的紫杉醇注射液,通过透射方式测定其在12000～4000cm-1的近红外吸收光谱,采用偏最小二乘算法(Partial least-squares,PLS)建立了校正模型,通过不同光谱预处理、维数、波数范围进行组合筛选。当采用减去一条直线,维数为6,波数范围为9002.5～4597.7cm-1时能最有效地提取光谱中的有效信息校正集标准偏差(Standard error of the calibration sets,SEC)为0.035,预测集标准偏差(Standard error of the prediction sets,SEP)为0.059,预测集样品的百分偏差都在±2%以内。该方法快速简便,适合于医院药房常用重要药品的快速测定分析。The objective of this study was to develop a method for the determination of taxol injection using near infrared transmission spectroscopy, turning out redetermination for injection from hospital pharmacy before using. Near infrared spectra （NIR） in the range of 12 000-4 000cm^-1 were recorded for the taxol injection manufactured during recent 24 mouths with different time. Calibration models were established using the partial least squares （PLS）. Comparing different spectra pretreatments methods, dimension and spectra range, The study showed that spectra information cab be extracted thoroughly by delete a line method with dimension 6, spectra range 9002.5 -4597. 7cm^-1, standard error of the calibration sets（SEC） 0. 035 and standard error of the prediction sets （SEP） 0. 059. Percent of prediction sets sample is less than ±2%. Results indicate that near infrared transmission spectroscopy method can be used to rapidly analyze the frequent and important drugs from hospital pharmacy.

关 键 词：近红外透射光谱 医院药房 紫杉醇注射液 偏最小二乘算法 

分 类 号：R927.2[医药卫生—药学]