蛋白改性纳米磁性颗粒的制备与性能研究  

Preparation and Characteristics of Iron Oxide Nanoparticles Modified by Bovine Serum Albumin

作　　者：赵鹏[1] 翁杰[1] 侯鹏[1] 

机构地区：[1]西南交通大学材料先进技术教育部重点实验室、材料科学与工程学院,成都610031

出　　处：《生物医学工程学杂志》2009年第5期1005-1009,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(50573026)

摘　　要：采用化学共沉淀法制备了纳米四氧化三铁(Fe3O4)磁性颗粒,然后用牛血清白蛋白对表面氨基化后的磁性颗粒进行了化学修饰。利用X-射线衍射、透射电子显微镜、红外光谱、接触角仪以及样品磁振动仪等手段对改性前后磁性颗粒的结构和性能进行了表征。结果表明,改性使纳米磁性颗粒具有良好的分散性,亲水性能大大增强,而饱和磁化强度变化不大,氨基硅烷的引入有利于提高蛋白在磁性颗粒表面的包覆效率,改性后的磁性颗粒可以作为靶向载体。Ultrafine iron oxide nanoparticles were prepared in aqueous solution using chemical coprecipitation method. Adsorption of bovine serum albumin（BSA） on magnetic particles was studied in the presence of silane coupling KH550. Characterizations of magnetic particles were carried out by X-ray diffraction, Transmission electron microscopy, Fourier transform infrared spectrometer and vibrating-sample magnetometer. The experimental results showed that magnetic nanoparticles were well dispersed with a small decrease of the magnetic saturation after modification. Magnetic nanoparticles wrapped in KH550 were favorable to the adsorption of BSA while the capability of hydrophile increased consequently, which could be used for target carrier in biomedicine.

关 键 词：纳米磁性颗粒 氨基硅烷 牛血清白蛋白 表面修饰 

分 类 号：TB383.1[一般工业技术—材料科学与工程]