肾血管性高血压大鼠肥厚心肌内中介素基因表达及缬沙坦、氨氯地平和依那普利的干预作用  

Intermedin (IMD) Gene Expression in Hypertrophic Cardiac Myocyte of Renal Vascular Hypertension Rats and the Intervention of Valsartan,Amlodipine and Enalapril in the Expression

作　　者：董劲[1] 陈晓平[1] 苏艳玲[1] 辛洪波[2] 蒋维[2] 蒋凌云[1] 

机构地区：[1]四川大学华西医院心内科,成都610041 [2]四川大学国家重点生物治疗实验室心血管病实验室I,成都610041

出　　处：《生物医学工程学杂志》2009年第5期1082-1087,共6页Journal of Biomedical Engineering

摘　　要：研究中介素(intermedin,IMD)基因在肾血管性高血压大鼠肥大心肌中的表达,以及缬沙坦、氨氯地平、依那普利三种不同药物降压后心肌中IMD基因表达的变化。用60只SD雄性大鼠随机分成假手术(作为对照组)和手术组,后者不完全性结扎左侧肾动脉,4周后又将手术组中成功建立肾性高血压模型的30只大鼠随机分为单纯结扎组和药物干预组,后者再分为三组,分别给于缬沙坦、氨氯地平、依那普利。药物干预10周后测定鼠尾动脉血压、心肌横径、左心室质量指数,RT-PCR半定量法检测心肌中IMD mRNA水平表达。结果显示单纯结扎组大鼠尾动脉收缩压较对照组明显升高,左室心肌横径、左室质量指数显著增加,左心室肌IMD mRNA表达也明显上调。缬沙坦、氨氯地平、依那普利干预组的血压、左室心肌横径、左室质量指数以及IMD mRNA表达显著低于单纯结扎组,但仍高于对照组,且三组间无显著差异。结论提示肾动脉不全结扎致高血压模型的肥大心肌中IMD mRNA得以上调,这可能改善左心室肥厚的病理生理过程,缬沙坦、氨氯地平和依那普利分别干预后,血压及肥大心肌改善,且心肌IMD mRNA表达均可以下调,提示心肌IMD mRNA的表达与血压或心肌肥大密切相关,未因降压药物的种类不同而表现出差异。This experiment on rats was aimed to investigate the expression of intermedin （IMD） in hypertrophic cardiac myoctye of renal vascular hypertension induced by incomplete ligation of the left renal artery, and so to detect and compare the changes of the expression after administration of Valsartan, Amlodipine and Enalapril respectively. The criterion for standard modeling was systolic pressure ≥ 140 mmHg. At 4 weeks after successful modeling, 60 SD male rats were randomly divided into 5 groups, namely the hypertrophy group, the 3 drug-treatment groups, and the sham-operation group as control. Blood pressure, left ventricular mass index（LVMI）, and the left ventricular mean transverse diameter of myocardial cell （LVTDM）were investigated at the 10th week after model establishment. Gene expression of IMD mRNA was detected by reverse transcription-polymerase chain reaction（RT-PCR）, and the optical density of the band was measured by use of the Gel Documentation System. The ratio of IMD mRNA to β-actin mRNA was considered the relative amount of IMD. When compared with control, the blood pressure increased significantly in the hypertrophy group. There was no statistically significant difference between the treatment groups. No significant difference in heart rate was noted at 4 weeks after operation in all groups. LVMI and LVTDM levels were significantly higher in the hypertrophy group than in the other groups; LVMI and LVTDM levels showed no significant difference among the treatment groups but they were obviously higher than those of the Sham-operation group. The gene expression of IMD mRNA in the hypertrophy group was upregulated in the myocardium, when compared with that in the other groups. Meanwhile, although IMD mRNA in the treament groups was higher than that in the Sham-operation group, no statistically significant difference of myocardial IMD mRNA was found between the treament groups. These results suggested that, in this experiment, intracardiac IMD mRNA was upregulated and could participat

关 键 词：中介素 肾血管性高血压 心肌肥厚 

分 类 号：R544.1[医药卫生—心血管疾病]