缺血后适应对老年冠心病患者缺血再灌注肢体血管内皮功能的影响  

Effect of ischemia preconditioning and postconditioning on emergency percutaneous coronary intervention in elderly patients with acute myocardial infarction

作　　者：马晓静[1] 齐春蕾[2] 张兴华[1] 马红军[2] 李春梅[1] 邵建华[1] 

机构地区：[1]山东大学附属省立医院心内科,山东济南250021 [2]山东省医学科学院附属医院

出　　处：《中国老年学杂志》2009年第19期2421-2423,共3页Chinese Journal of Gerontology

基　　金：山东省医药卫生科研基金资助项目(2003HZ105);山东省自然科学基金资助项目(Y2005C70)

摘　　要：目的观察缺血再灌注不同时刻给予缺血后适应,对老年冠心病患者血管内皮功能的保护作用。方法选取老年冠心病患者54例,随机分为3组,缺血再灌注组、后适应组及延迟后适应组。建立肢体缺血再灌注模型,在再灌注不同时刻(1min内及1min后)分别给予后适应组及延迟后适应组缺血后适应干预,通过超声检测肱动脉血流介导的舒张功能(FMD),观察缺血再灌注前后反应性充血血管内径的变化。结果缺血再灌注组再灌注后FMD明显减小(P<0.05),再灌注1min内给予缺血后适应有明显的血管内皮功能保护作用,与缺血再灌注组比较,后适应组FMD明显提高(6.70±2.36vs3.05±0.91,P<0.05),而延迟后适应组则失去了内皮功能保护作用(3.17±1.04vs3.05±0.91,P>0.05)。结论再灌注1min内给予缺血后适应可以改善血管内皮功能,但延迟后适应的保护作用消失。Objective To express human Y2 receptor protein in E. coli,purify and identify it,and conduct bioinformatic analysis of Y2 receptor protein. Methods The recombinant plasmid pET28a-Y2 which had been well constructed and sequentially confirmed was transplanted into E. coli BL21 （ DE3 ） and induced by IPTG to express fusion proteins. SDS-PAGE and Western blot were used to test and identify the expressed fusion proteins. The inclusion body of the expressed product was purified by Ni^2＋-NTA affinity chromatography. Then bioinformatic analysis of the Y2 receptor was conducted with the help of related online software. Results After being induced by IPTG, the DE3 with recombinant plasmid pET28a-Y2 expressed recombinant human Y2 receptor protein. Highly purified fusion protein was obtained by Ni^2＋-NTA affinity chromatography. Related biological characteristics of Y2 receptor were obtained after the online software analysis. Conclusions The recombinant plasmid pET28a-Y2 can be successfully expressed in DE3. Highly purified proteins can be obtained by Ni^2＋-NTA affinity chromatography. Y2 receptor＇s biological characteristics are predicted, which lays foundation for further studies of Y2 receptor protein＇s biological function and antibody development.

关 键 词：冠心病 再灌注损伤 缺血后适应 血管内皮 延迟后适应 

分 类 号：R541.4[医药卫生—心血管疾病]