骨质疏松大鼠的骨形成蛋白-4诱导成骨信号转导机制变化  

Signal transduction mechanism change of bone morphogenetic protein-4(BMP-4) in osteoporosis

作　　者：胡静[1] 郑洪新[2] 

机构地区：[1]上海中医药大学温病教研室,上海201203 [2]辽宁中医药大学基础医学院

出　　处：《中国老年学杂志》2009年第19期2462-2464,共3页Chinese Journal of Gerontology

基　　金：辽宁省自然科学基金资助项目(2001101029)

摘　　要：目的观察骨形成蛋白4(BMP-4)及其信号转导蛋白(Smad)5、6在去卵巢大鼠骨组织中的表达。方法采用切除大鼠双侧卵巢法复制骨质疏松模型,术后94d,用双能X线骨密度仪检测骨密度;用Western印迹法检测骨组织中BMP-4和Smad5、6蛋白表达水平。结果与正常组比较,模型空白组大鼠股骨骨密度显著下降(P<0.01);BMP-4和Smad5蛋白表达水平显著降低(P<0.01),Smad6蛋白表达水平明显升高(P<0.01)。结论骨组织中BMP-4、Smad5蛋白表达水平的下降和Smad6蛋白表达水平的升高,可能是骨质疏松发生的重要机制之一。Objective To study the expression and significance of the bone morphogenetic protein-4 （BMP-4） and signal transduction protein Smad5, 6 in the bone tissue of ovariectomized rat. Methods The osteoporosis （OP） model rat was established by removal bilateral ovaries. Bone mineral density （BMD） was measured by Dual-Energy X Ray, BMP-4, Smad5,6 protein were measured by Western blotting on 94 days after surgery. Results Compared with the normal rats, the BMD of femoral bone in vitro in model group decreased obviously （ P 〈 0. 01 ）, the expressions of BMP-4 and Smad5 protein decreased obviously（ P 〈 0.01 ） and the expression of Smad6 protein increased obviously （ P 〈 0. 01 ） in model group. Conclusions Conclusions The decrease of BMP-4 and Smad5 and the increase of Smad6 in bone tissue may be one of the mechanisms of postmenopausal OP.

关 键 词：骨质疏松 骨形成蛋白-4 信号转导 SMADS蛋白 

分 类 号：R58[医药卫生—内分泌]