二黄糖肾康对糖尿病肾病大鼠肾脏细胞凋亡及PI3 K/AKT信号转导系统的影响  

Effect of Erhuangtangshenkang on diabetic nephopathy rat kidney cell apoptosis and PI3 K/AKT signal transduction system

作　　者：常庚[1] 成秀梅[2] 潘莉[2] 梁文杰[2] 张金虎[2] 常风云[2] 

机构地区：[1]河北医科大学第二医院,河北石家庄050001 [2]河北医科大学中西医结合学院

出　　处：《中国老年学杂志》2009年第19期2471-2473,共3页Chinese Journal of Gerontology

基　　金：河北省卫生厅资助课题(05046)

摘　　要：目的观察二黄糖肾康对糖尿病肾病(DN)大鼠细胞凋亡及PI3 K/AKT信号转导系统的影响。方法SD大鼠单侧肾切除加腹腔注射链脲佐菌素(STZ)复制DN模型,每日二黄糖肾康灌胃,8w后流式细胞术检测细胞凋亡数目及调控因子Bax、Bcl-2、Fas、Fas-L的蛋白表达,采用Western印迹方法检测细胞内磷酸化Akt蛋白质的表达。结果二黄糖肾康明显减少DN大鼠肾脏皮质细胞凋亡数目,降低Bax、Fas、Fas-L的的蛋白表达,增加Bcl-2的蛋白表达,激活PI3K/AKT信号通路。结论二黄糖肾康能有效降低肾脏细胞凋亡,激活PI3K/AKT信号通路。Objective To observe the effect of Erhuangtangshenkang on diabetic nephopathy （DN） rat kidney cell apoptosis and PI3 K/AKT signal transduction system. Methods DN model rats were made by streptozotocin （STZ） lumbar injection and unilateral nephrectomy with SD rats and were feed with Erhuangtangshenkang gastric perfusion. Cell apoptosis rate and the expressions of Bax, Bcl-2, Fas and Fas-L were detected by flow cytometry. The expression of phosphorylation Akt protein was measured by Western blotting. Results Erhuangtangshenkang could decrease the number of apoptosis cell in DN rat＇s renal cortex, reduce the expressions of Bax, Fas and Fas-L, increase the expression of Bcl-2 and activate P13 K/AKT signal pathway. Conclusions Erhuangtangshenkang can efficiently reduce kidney cell apoptosis and activate PI3 K/AKT signal pathway.

关 键 词：二黄糖肾康 DN 细胞凋亡 Bax Bcl-2 Fas FAS-L PI3K/AKT 

分 类 号：R285.5[医药卫生—中药学]