次黄嘌呤分子印迹电化学传感器的研制  

Preparation of electrochemical sensor for hypoxanthine by molecular imprinted polymer

作　　者：王志华[1] 陈义文[1] 张会妮[1] 李晓宁[1] 康敬万[1] 

机构地区：[1]西北师范大学化学化工学院,甘肃兰州730070

出　　处：《化学研究与应用》2009年第10期1370-1374,共5页Chemical Research and Application

基　　金：甘肃省高分子材料重点实验室资助项目

摘　　要：以丙烯酰胺为功能性单体，N，N’-亚甲基双丙烯酰胺为交联剂，次黄嘌呤为模板分子，在pH13．5的强碱性溶液中采用循环伏安法合成了聚丙烯酰胺膜，以此为识别元件制备了次黄嘌呤电容型生物传感器，并用电化学阻抗技术表征了电聚合分子印迹膜的性能。在2—15μmol／L的范围之内，次黄嘌呤浓度的变化与电容的变化成线性关系。每次加入次黄嘌呤后响应约在5min后达到稳定，传感器制备容易，检测快速。与传统的聚丙烯酰胺膜不同，该膜在碱性溶液中制备，稳定性好，不易溶胀。Using acrylamide as functional monomer, N, N＇ -methylene diacrylamide as the cross linking agent, hypoxanthine as template, molecular imprinted polymer membrane is synthesized in pH = 13.5 solution by cyclic vohammetry. The characteristics of the polymer are studied by cyclic vohammetry and a. c. impedence technology. Linear range is 2 - 15μmol/L and the responding time is 5 min. The sensors so formed possess easily prepared and detection speediness. Comparing with the traditional polyacrylamide,the membrane we prepared in basic solution has good stability.

关 键 词：次黄嘌呤 丙烯酰胺 分子印迹膜 

分 类 号：O657.1[理学—分析化学]