不同氮素水平下冬小麦叶片酚酸类物质代谢对FACE的响应  

Effect of Free Air CO_2 Enrichment (FACE) on phenolic acid metabolism in winter wheat leaf under different nitrogen levels

作　　者：曹际玲[1,2] 朱建国[1] 马红亮[1,2] 朱春梧[1,2] 颜建[1] 曾青[1] 寇太记[1,2] 谢祖彬[1] 

机构地区：[1]中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室,南京210008 [2]中国科学院研究生院,北京100080

出　　处：《中国生态农业学报》2009年第5期837-841,共5页Chinese Journal of Eco-Agriculture

基　　金：国家自然科学基金面上项目(30470334;30770408);国家自然科学基金重点项目(40231003);中国科学院创新方向项目(KZXC3-SW-44O);国家重点基础研究发展计划(973计划)项目(2002CB412502)资助

摘　　要：利用开放式空气CO2浓度升高（Free Air Carbon-dioxide Enrichment,FACE）平台,研究了低氮（LN）和常氮（NN）水平下,大气CO2浓度升高对冬小麦叶片酚酸类物质代谢的影响。结果表明,CO2浓度升高对小麦叶片水杨酸、对羟基苯甲酸、肉桂酸、阿魏酸和香草酸含量的影响随供氮水平的不同而有所差异。低氮下小麦通过提高叶片苯丙氨酸解氨酶（PAL）活性（30.1%）而使其含量均显著增加,增幅分别达33.7%、119.6%、26.7%、39.9%和28.6%;而常氮下PAL活性和酚酸类含量变化均未达显著水平。可见,大气CO2浓度升高对冬小麦酚酸类物质代谢的影响受氮水平的调控,在未来CO2浓度升高条件下,选择适宜的施肥水平将显得更为重要。此外,总酚含量与水杨酸、对羟基苯甲酸、肉桂酸、阿魏酸和香草酸等含量变化趋势基本一致,且总酚含量变化的79.6%~151.4%是由这几种酚酸含量变化引起的,说明CO2浓度升高使水杨酸、对羟基苯甲酸、肉桂酸、阿魏酸和香草酸等含量增加是总酚含量增加的直接原因。低氮条件下大气CO2浓度升高将通过改变酚酸类物质代谢而间接影响小麦与伴生杂草的关系。Effect of elevated pCO2 on phenolic acid metabolism in the leaves of winter wheat under low N （150 kg·hm-2） and normal N （250 kg·hm-2） levels was determined via FACE.The results show different impacts of elevated pCO2 on the contents of salicylic acid,p-Hydroxybenzoic acid,cinamic acid,ferulic acid and vanillic acid,as well as phenylalnine ammonialyase （PAL） activity in winter wheat leaves under low N and normal N levels.At 1ow N level,salicylic acid,p-Hydroxybenzoic acid,cinamic acid,ferulic acid and vanillic acid are respectively significantly enhanced by 33.7%,119.6%,26.7%,39.9% and 28.6% due to 30.9% increase in phenylalnine ammonialyase activity under elevated pCO2.This,however,does not change significantly under normal N level.Hence the influence of elevated pCO2 on phenolic acid metabolism in winter wheat leaf depends on N supply.It is highly important to determine appropriate fertilization rate in elevated CO2 conditions.Furthermore,total phenol content shows the same variation trend as that of salicylic acid,p-Hydroxybenzoic acid,cinamic acid,ferulic acid and vanillic acid;and 79.6%～151.4% total phenol variation is caused by these acids changes.It then suggests that total phenol content increase in winter wheat leafis partly accounted for enhanced phenolic acid metabolism under elevated pCO2.Under low N level,the rise in atmospheric CO2 may indirectly affect wheat-weed relationship by changing phenolic acid metabolism.

关 键 词：CO2浓度升高 氮水平 小麦 化感物质 总酚 酚酸类物质 苯丙氨酸解氨酶(PAL) 

分 类 号：Q948.11[生物学—植物学]