泽泻防治泌尿系结石有效部位提取工艺研究  

Screening Study on Extraction Technique for Urinary Calculi Preventive Components from Rhizoma Alismatis

作　　者：刘妙娜[1] 贺战梅 张立冬[1] 林智诚[1] 严书超[1] 区淑蕴[1] 尹春萍[1] 

机构地区：[1]华中科技大学同济医学院药学院天然药物化学与资源品质评价湖北省重点实验室,武汉430030 [2]武汉钢铁集团总公司第二职工医院药剂科,430085

出　　处：《医药导报》2009年第10期1329-1331,共3页Herald of Medicine

基　　金：武汉市科技攻关资助项目(基金编号:200960323114)

摘　　要：目的筛选泽泻防治泌尿系结石有效部位的最佳提取工艺。方法采用正交实验设计,应用HPLC-ELSD法测定泽泻提取物中23-乙酰泽泻醇B含量,并以23-乙酰泽泻醇B含量为考察指标,考察甲醇浓度、甲醇倍数、提取次数、提取时间等4种因素对提取结果的影响。结果泽泻防治泌尿系结石有效部位的最佳提取工艺为加入8倍量50%甲醇,提取3次,每次1.5 h。结论该提取工艺合理、可行。Objective To screen the optimal extraction technique for the urinary calculi prevention components from rhizome alismatis. Methods The content of Alisol B 23-acetate in the extraction of rhizome alismatis was determined by using HPLC-EISD and used as a marker to investigate effects of 4 factors： methanol concentration, extraction time, extraction frequency, and methanol dosage on components extraction with an orthogonal test. Results The optimal extraction technique for effective components from rhizome alismatis was to extract the medicinal material with 8 times amount of 50% methanol for 3 times, 1.5 hours for each. Conclusion The extraction technique is reasonable and practicable.

关 键 词：泽泻 泽泻醇B-23乙酸酯 正交实验 HPLC—ELSD法 

分 类 号：R282.71[医药卫生—中药学] TQ460.6[医药卫生—中医学]