用放射免疫测定法分析土壤中莠去津残留量的研究  

ANALYSIS ATRAZINE RESIDUES IN SOILS USING RADIOIMMUNOASSAY

作　　者：徐德武[1,2,3] 杨根海[1,2,3] 秦凤琴 徐坤[1,2,3] 阎美玉 

机构地区：[1]山东省莱阳农学院 [2]中国农业大学基础科技学院 [3]山东省莱阳市环保局

出　　处：《核农学报》1998年第5期309-313,共5页Journal of Nuclear Agricultural Sciences

基　　金：联合国粮农组织和国际原子能机构联合处资助

摘　　要：根据莠去津均三氮苯类结构的特征，合成连有活性羧基的莠去津半抗原，通过活性脂法与牛血清白蛋白联接，制备出较高生物活性的人工抗原，以此免疫兔子获得抗莠去津的多克隆抗体。抗体的特异性强，与扑灭津的交叉反应性为９３％，与西玛津的交叉反应性为８％。以３Ｈ标记莠去津建立的莠去津放射免疫测定法对土壤中莠去津添加回收率的测定表明，沙土的添加回收率为８５７％～９５０％，壤土的添加回收率为８６９％～９２１％。The determination of atrazine residue in soil with radioimmunoassay method was studied. The high bioactive antigen was synthesized and the high titer anti atrazine antibody was pre pared. The antiserum was specific for atrazine, the cross reactivity of the antiserum to propazine and simazine was 93% and 8%, respectively. The RIA for atrazine labeled by 3H was established and showed that the rate of recovery by adding atrazine in sand and clay soils were 89 7%～95 0% and 86 9%～92 1%, that the recoveries of atrazine in sand and clay soil were 85 7%～95 0% and 86 9%～92 1%,respectively.

关 键 词：除草剂 莠去津 放射免疫测定法 土壤 残留量 

分 类 号：S482.49[农业科学—农药学] X592[农业科学—植物保护]